The TOG5 domain of CKAP5 is required to interact with F-actin and promote microtubule advancement in neurons
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F24%3A00602186" target="_blank" >RIV/86652036:_____/24:00602186 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10489383
Result on the web
<a href="https://www.molbiolcell.org/doi/abs/10.1091/mbc.E24-05-0202" target="_blank" >https://www.molbiolcell.org/doi/abs/10.1091/mbc.E24-05-0202</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1091/mbc.E24-05-0202" target="_blank" >10.1091/mbc.E24-05-0202</a>
Alternative languages
Result language
angličtina
Original language name
The TOG5 domain of CKAP5 is required to interact with F-actin and promote microtubule advancement in neurons
Original language description
Microtubule (MT) and F-actin cytoskeletal cross-talk and organization are important aspects of axon guidance mechanisms, but how associated proteins facilitate this function remains largely unknown. While the MT-associated protein, CKAP5 (XMAP215/chTOG), has been best characterized as a MT polymerase, we have recently highlighted a novel role for CKAP5 in facilitating interactions between MT and F-actin in vitro and in embryonic Xenopus laevis neuronal growth cones. However, the mechanism by which it does so is unclear. Here, using in vitro reconstitution assays coupled with total internal reflection fluorescence microscopy, we report that the TOG5 domain of CKAP5 is necessary for its ability to bind to and bundle actin filaments, as well as to cross-link MTs and F-actin in vitro. Additionally, we show that this novel MT/F-actin cross-linking function of CKAP5 is possible even in MT polymerase-incompetent mutants of CKAP5 in vivo. Indeed, CKAP5 requires both MT and F-actin binding, but not MT polymerization, to promote MT-F-actin alignment in growth cones and axon outgrowth. Taken together, our findings provide mechanistic insights into how MT populations penetrate the growth cone periphery through CKAP5facilitated interaction with F-actin during axon outgrowth and guidance.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Biology of the Cell
ISSN
1059-1524
e-ISSN
1939-4586
Volume of the periodical
35
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
br24
UT code for WoS article
001363454400003
EID of the result in the Scopus database
2-s2.0-85210453581