Excited-State Mixing in the LOV Domain Proteins: Possible Physics behind the Difference in the Transient Absorption and Transient Stimulated Raman Spectroscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F25%3A00619306" target="_blank" >RIV/86652036:_____/25:00619306 - isvavai.cz</a>
Alternative codes found
RIV/10974938:_____/25:25_88_04
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.jpclett.4c02978" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.jpclett.4c02978</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jpclett.4c02978" target="_blank" >10.1021/acs.jpclett.4c02978</a>

Result language
angličtina
Original language name
Excited-State Mixing in the LOV Domain Proteins: Possible Physics behind the Difference in the Transient Absorption and Transient Stimulated Raman Spectroscopy
Original language description
It remains uncertain whether excited electronic state mixing occurs in the flavin cofactor of the light-oxygen-voltage-sensing (LOV) domain. In this study, we present transient absorption and femtosecond stimulated Raman spectra of both free and EL222 binding flavin mononucleotide (FMN). We observed a change in the shape of the excited-state absorption around 800 nm in the S1 state transient absorption after binding to EL222, alongside a relative intensity increase of the N1-C2 and C2=O2 stretching modes in the S1 state Raman spectra. Based on the previous calculated geometric differences between the pi pi* and n pi* states, we propose a probable electronic state mixing in EL222 binding FMN. This mixing is favored by the nonsymmetric hydrogen bonding interaction between the flavin O4 atom and the asparagine residue and fewer hydrogen bonds with the O2 atom in EL222.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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