Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F16%3A00467450" target="_blank" >RIV/86652079:_____/16:00467450 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17344/acsi.2016.2484" target="_blank" >http://dx.doi.org/10.17344/acsi.2016.2484</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17344/acsi.2016.2484" target="_blank" >10.17344/acsi.2016.2484</a>

Result language

angličtina

Original language name

Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues

Original language description

Physiological studies in plants often require enzyme extraction from tissues containing high concentrations of phenols and polyphenols. Unless removed or neutralized, such compounds may hinder extraction, inactivate enzymes, and interfere with enzyme detection. The following protocol for activity assays for enzymes of primary carbohydrate metabolism, while based on our recently published one for quantitative measurement of activities using coupled spectrophotometric assays in a 96-well format, is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grapevine leaf. As a case study we applied the protocol to grapevine leaf samples infected with plant pathogenic bacteria 'Candidatus Phytoplasma solani', known to alter carbohydrate metabolism in grapevine. The described adaptations may be useful for determination of metabolic fingerprints for physiological phenotyping of other plant species with inherently high levels of phenolic compounds. nn

Czech name

—

Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EH - Ecology - communities

OECD FORD branch

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Project

<a href="/en/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 – Development of the Centre of Global Climate Change Impacts Studies</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Chimica Slovenica

ISSN

1318-0207

e-ISSN

—

Volume of the periodical

63

Issue of the periodical within the volume

4

Country of publishing house

SI - SLOVENIA

Number of pages

6

Pages from-to

757-762

UT code for WoS article

000392743500009

EID of the result in the Scopus database

2-s2.0-85003875024