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Stress Response Monitoring of Photoautotrophic Higher Plant Suspension Cultures by Fluorescence Imaging for High-Throughput Toxic Compound Screening

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F17%3A00476138" target="_blank" >RIV/86652079:_____/17:00476138 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.4236/jep.2017.86044" target="_blank" >http://dx.doi.org/10.4236/jep.2017.86044</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.4236/jep.2017.86044" target="_blank" >10.4236/jep.2017.86044</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Stress Response Monitoring of Photoautotrophic Higher Plant Suspension Cultures by Fluorescence Imaging for High-Throughput Toxic Compound Screening

  • Original language description

    Photoautotrophic suspension cultures have been established from various model and crop plants and proved to be valuable and robust experimental system to assess coordinated responses of primary and secondary metabolism to metabolic and stress related signals. The use of suspension cultures combines the ease of handling microalgae in microtiter plates with the advantage of testing physiological responses of higher plants, notably in combination with the assessment of the response of photosynthetic activity by PAM chlorophyll fluorescence imaging as well as monitoring changes in secondary metabolite production and ROS formation by steady state fluorescence of plant fluorophores or introduced fluorescent probes. Photoautotrophic cultures provide various advantages as fast, highly sensitive, robust and high-through-put experimental system for screening and characterization of the impact of toxic compounds on higher plants. This opinion article discusses and critically evaluates the potential of photoautotrophic cultures of higher plants in combination with fluorescence imaging assays in microtiter plates as a complement to existing guidelines for testing the toxicity of chemicals in plants.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>ost</sub> - Miscellaneous article in a specialist periodical

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Environmental Protection

  • ISSN

    2152-2197

  • e-ISSN

  • Volume of the periodical

    8

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    CN - CHINA

  • Number of pages

    15

  • Pages from-to

    678-692

  • UT code for WoS article

  • EID of the result in the Scopus database