A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F19%3A00495975" target="_blank" >RIV/86652079:_____/19:00495975 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/19:00112324

Result on the web

<a href="https://www.sciencedirect.com/science/article/abs/pii/S1386142518308886?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1386142518308886?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.saa.2018.09.036" target="_blank" >10.1016/j.saa.2018.09.036</a>

Result language

angličtina

Original language name

A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum

Original language description

Lichens are successful colonizers in extreme environments worldwide, and they are considered to have played an important role during the evolution of life. Here, we have used a correlative approach, combining three optical signals (chlorophyll a fluorescence (ChlF), reflectance, and Raman spectra), to monitor hydration induced changes in photosynthetic properties of an Antarctic chlorolichen Dermatocarpon polyphyllizum. We measured these three signals from this lichen at different stages (after 4 h, 24 h, and 48 h) of hydration, and compared the data obtained from this lichen in “dry state” as well as in different “hydrated state”. We found that dry state of this lichen has: (1) no variable ChlF, (2) high reflectance, with no red-edge and almost zero photochemical reflectance index (PRI), and (3) low-intensity Raman bands of their carotenoids. Furthermore, 4 h of hydration, increased its relative water content (RWC) by 93%, showed red-edge in reflectance spectra, and changed the maximum quantum yield of PSII photochemistry (Fv/Fm) from 0 to 0.57 ± 0.01. We found that reflectance indices, normalized difference index (NDVI) and PRI, significantly differed between brown and black/green surface areas, at all hydration stages, whereas, a shift in the Raman ν1(C[dbnd]C) band, between brown and black/green surface areas, occurred in 24 h or 48 h hydrated samples. These data indicate that hydration shortly (within 4 h) activated functions of photosynthetic apparatus, and the de novo synthesis of carotenoids occured in 24 h or 48 h. Furthermore, exposure to high irradiance (2000 μmol photons m−2s−1), in 48 h hydrated lichen, significantly reduced Fv/Fm(signifies photoinhibition) and increased PRI (represents changes in xanthophyll pigments). We conclude that the implication of such a correlative approach is highly useful for understanding survival and protective mechanisms on extremophile photosynthetic organisms.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10618 - Ecology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

ISSN

1386-1425

e-ISSN

—

Volume of the periodical

208

Issue of the periodical within the volume

feb

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

13-23

UT code for WoS article

000449900700003

EID of the result in the Scopus database

2-s2.0-85054147877