Lipid-polymorphism of plant thylakoid membranes.Enhanced non-bilayer lipid phases associated with increased membrane permeability
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F19%3A00504213" target="_blank" >RIV/86652079:_____/19:00504213 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppl.12929" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppl.12929</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ppl.12929" target="_blank" >10.1111/ppl.12929</a>
Alternative languages
Result language
angličtina
Original language name
Lipid-polymorphism of plant thylakoid membranes.Enhanced non-bilayer lipid phases associated with increased membrane permeability
Original language description
Earlier experiments, using 31P‐NMR and time‐resolved merocyanine fluorescence spectroscopy, have shown that isolated intact, fully functional plant thylakoid membranes, in addition to the bilayer phase, contain three non‐bilayer (or non‐lamellar) lipid phases (Garab et al. 2017 Scientific Reports 7: 13343). It has also been shown that the lipid polymorphism of thylakoid membranes can be characterized by remarkable plasticity, i.e. by significant variations in 31P‐NMR signatures. However, changes in the lipid‐phase behavior of thylakoids could not be assigned to changes in the overall membrane organization and the photosynthetic activity, as tested by circular dichroism and 77 K fluorescence emission spectroscopy and the magnitude of the variable fluorescence of photosystem II, which all showed only marginal variations. In this work, we investigated in more detail the temporal stability of the different lipid phases by recording 31P‐NMR spectra on isolated thylakoid membranes that were suspended in sorbitol‐ or NaCl‐based media. We observed, at 5°C during 8 hours in the dark, substantial gradual enhancement of the isotropic lipid phases and diminishment of the bilayer phase in the sorbitol‐based medium. These changes compared well with the gradually increasing membrane permeability, as testified by the gradual acceleration of the decay of flash‐induced electrochromic absorption changes and characteristic changes in the kinetics of fast chlorophyll a‐fluorescence transients, all variations were much less pronounced in the NaCl‐based medium. These observations suggest that non‐bilayer lipids and non‐lamellar lipid phases play significant roles in the structural dynamics and functional plasticity of thylakoid membranes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 – Development of the Centre of Global Climate Change Impacts Studies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiologia Plantarum
ISSN
0031-9317
e-ISSN
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Volume of the periodical
166
Issue of the periodical within the volume
may
Country of publishing house
DK - DENMARK
Number of pages
10
Pages from-to
278-287
UT code for WoS article
000466108300022
EID of the result in the Scopus database
2-s2.0-85062711237