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Lipid-polymorphism of plant thylakoid membranes.Enhanced non-bilayer lipid phases associated with increased membrane permeability

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F19%3A00504213" target="_blank" >RIV/86652079:_____/19:00504213 - isvavai.cz</a>

  • Result on the web

    <a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppl.12929" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppl.12929</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/ppl.12929" target="_blank" >10.1111/ppl.12929</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Lipid-polymorphism of plant thylakoid membranes.Enhanced non-bilayer lipid phases associated with increased membrane permeability

  • Original language description

    Earlier experiments, using 31P‐NMR and time‐resolved merocyanine fluorescence spectroscopy, have shown that isolated intact, fully functional plant thylakoid membranes, in addition to the bilayer phase, contain three non‐bilayer (or non‐lamellar) lipid phases (Garab et al. 2017 Scientific Reports 7: 13343). It has also been shown that the lipid polymorphism of thylakoid membranes can be characterized by remarkable plasticity, i.e. by significant variations in 31P‐NMR signatures. However, changes in the lipid‐phase behavior of thylakoids could not be assigned to changes in the overall membrane organization and the photosynthetic activity, as tested by circular dichroism and 77 K fluorescence emission spectroscopy and the magnitude of the variable fluorescence of photosystem II, which all showed only marginal variations. In this work, we investigated in more detail the temporal stability of the different lipid phases by recording 31P‐NMR spectra on isolated thylakoid membranes that were suspended in sorbitol‐ or NaCl‐based media. We observed, at 5°C during 8 hours in the dark, substantial gradual enhancement of the isotropic lipid phases and diminishment of the bilayer phase in the sorbitol‐based medium. These changes compared well with the gradually increasing membrane permeability, as testified by the gradual acceleration of the decay of flash‐induced electrochromic absorption changes and characteristic changes in the kinetics of fast chlorophyll a‐fluorescence transients, all variations were much less pronounced in the NaCl‐based medium. These observations suggest that non‐bilayer lipids and non‐lamellar lipid phases play significant roles in the structural dynamics and functional plasticity of thylakoid membranes.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10611 - Plant sciences, botany

Result continuities

  • Project

    <a href="/en/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 – Development of the Centre of Global Climate Change Impacts Studies</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Physiologia Plantarum

  • ISSN

    0031-9317

  • e-ISSN

  • Volume of the periodical

    166

  • Issue of the periodical within the volume

    may

  • Country of publishing house

    DK - DENMARK

  • Number of pages

    10

  • Pages from-to

    278-287

  • UT code for WoS article

    000466108300022

  • EID of the result in the Scopus database

    2-s2.0-85062711237