Spatial and Temporal Variability of Plant Leaf Responses Cascade after PSII Inhibition: Raman, Chlorophyll Fluorescence and Infrared Thermal Imaging

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F20%3A00523912" target="_blank" >RIV/86652079:_____/20:00523912 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/1424-8220/20/4/1015" target="_blank" >https://www.mdpi.com/1424-8220/20/4/1015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s20041015" target="_blank" >10.3390/s20041015</a>

Result language

angličtina

Original language name

Spatial and Temporal Variability of Plant Leaf Responses Cascade after PSII Inhibition: Raman, Chlorophyll Fluorescence and Infrared Thermal Imaging

Original language description

The use of photosystem II (PSII) inhibitors allows simulating cascade of defense and damage responses, including the oxidative stress. In our study, PSII inhibiting herbicide metribuzin was applied to the leaf of the model plant species Chenopodium album. The temporally and spatially resolved cascade of defense responses was studied noninvasively at the leaf level by combining three imaging approaches: Raman spectroscopy as a principal method, corroborated by chlorophyll a fluorescence (ChlF) and infrared thermal imaging. ChlF imaging show time-dependent transport in acropetal direction through veins and increase of area affected by metribuzin and demonstrated the ability to distinguish between fast processes at the level of electron transport (1 V-j) from slow processes at the level of non-photochemical energy dissipation (NPQ) or maximum efficiency of PSII photochemistry (Fv/Fm). The high-resolution resonance Raman images show zones of local increase of carotenoid signal 72 h after the herbicide application, surrounding the damaged tissue, which points to the activation of defense mechanisms. The shift in the carotenoid band indicates structural changes in carotenoids. Finally, the increase of leaf temperature in the region surrounding the spot of herbicide application and expanding in the direction to the leaf tip proves the metribuzin effect on slow stomata closure.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/LTC18036" target="_blank" >LTC18036: Advanced Raman imaging of plant and microalgae biomolecular response to environmental stress factors</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors

ISSN

1424-8220

e-ISSN

1424-8220

Volume of the periodical

20

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

1015

UT code for WoS article

000522448600066

EID of the result in the Scopus database

2-s2.0-85079572248