Regulation of Phenolic Compound Production by Light Varying in Spectral Quality and Total Irradiance
Result description
Photosynthetically active radiation (PAR) is an important environmental cue inducing the production of many secondary metabolites involved in plant oxidative stress avoidance and tolerance. To examine the complex role of PAR irradiance and specific spectral components on the accumulation of phenolic compounds (PheCs), we acclimated spring barley (Hordeum vulgare) to different spectral qualities (white, blue, green, red) at three irradiances (100, 200, 400 mu mol m(-2) s(-1)). We confirmed that blue light irradiance is essential for the accumulation of PheCs in secondary barley leaves (in UV-lacking conditions), which underpins the importance of photoreceptor signals (especially cryptochrome). Increasing blue light irradiance most effectively induced the accumulation of B-dihydroxylated flavonoids, probably due to the significantly enhanced expression of the F3 ' H gene. These changes in PheC metabolism led to a steeper increase in antioxidant activity than epidermal UV-A shielding in leaf extracts containing PheCs. In addition, we examined the possible role of miRNAs in the complex regulation of gene expression related to PheC biosynthesis.
Keywords
barley hordeum-vulgaregene-expressiongreen lightred-lightanthocyanin biosynthesishydroxycinnamic acidsantioxidant activitymediated regulationoxidative stressfunctional rolesantioxidantsflavonoidshplcmiRNAphotoprotectionsecondary metabolismspectral quality of lightspring barley (Hordeum vulgare)transcriptomicsUV tolerance
The result's identifiers
Result code in IS VaVaI
Alternative codes found
RIV/61988987:17310/22:A2302FYO RIV/61988987:17310/22:N2302FYO RIV/00216208:11310/22:10445330
Result on the web
DOI - Digital Object Identifier
Alternative languages
Result language
angličtina
Original language name
Regulation of Phenolic Compound Production by Light Varying in Spectral Quality and Total Irradiance
Original language description
Photosynthetically active radiation (PAR) is an important environmental cue inducing the production of many secondary metabolites involved in plant oxidative stress avoidance and tolerance. To examine the complex role of PAR irradiance and specific spectral components on the accumulation of phenolic compounds (PheCs), we acclimated spring barley (Hordeum vulgare) to different spectral qualities (white, blue, green, red) at three irradiances (100, 200, 400 mu mol m(-2) s(-1)). We confirmed that blue light irradiance is essential for the accumulation of PheCs in secondary barley leaves (in UV-lacking conditions), which underpins the importance of photoreceptor signals (especially cryptochrome). Increasing blue light irradiance most effectively induced the accumulation of B-dihydroxylated flavonoids, probably due to the significantly enhanced expression of the F3 ' H gene. These changes in PheC metabolism led to a steeper increase in antioxidant activity than epidermal UV-A shielding in leaf extracts containing PheCs. In addition, we examined the possible role of miRNAs in the complex regulation of gene expression related to PheC biosynthesis.
Czech name
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Czech description
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Classification
Type
Jimp - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Volume of the periodical
23
Issue of the periodical within the volume
12
Country of publishing house
CH - SWITZERLAND
Number of pages
30
Pages from-to
6533
UT code for WoS article
000815914400001
EID of the result in the Scopus database
2-s2.0-85131586192
Basic information
Result type
Jimp - Article in a specialist periodical, which is included in the Web of Science database
OECD FORD
Biochemistry and molecular biology
Year of implementation
2022