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Characterizing isoprene production in cyanobacteria-Insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F23%3A00572613" target="_blank" >RIV/86652079:_____/23:00572613 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/23:00572613

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S0960852423004947?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0960852423004947?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.biortech.2023.129068" target="_blank" >10.1016/j.biortech.2023.129068</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Characterizing isoprene production in cyanobacteria-Insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803

  • Original language description

    Engineering cyanobacteria for the production of isoprene and other terpenoids has gained increasing attention in the field of biotechnology. Several studies have addressed optimization of isoprene synthesis in cyanobacteria via enzyme and pathway engineering. However, only little attention has been paid to the optimization of cultivation conditions. In this study, an isoprene-producing strain of Synechocystis sp. PCC 6803 and two control strains were grown under a variety of cultivation conditions. Isoprene production, as quantified by modified membrane inlet mass spectrometer (MIMS) and interpreted using Flux Balance Analysis (FBA), increased under violet light and at elevated temperature. Increase of thermotolerance in the isoprene producer was attributed to the physical presence of isoprene, similar to plants. The results demonstrate a beneficial effect of isoprene on cell survival at higher temperatures. This increased thermotolerance opens new possibilities for sustainable bio-production of isoprene and other products.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/EF16_026%2F0008413" target="_blank" >EF16_026/0008413: Strategic Partnership for Environmental Technologies and Energy Production</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Bioresource Technology

  • ISSN

    0960-8524

  • e-ISSN

    1873-2976

  • Volume of the periodical

    380

  • Issue of the periodical within the volume

    JUL 2023

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    10

  • Pages from-to

    129068

  • UT code for WoS article

    000991095500001

  • EID of the result in the Scopus database

    2-s2.0-85153498734