Review of embedding and non-embedding techniques for quantitative wood anatomy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F24%3A00597718" target="_blank" >RIV/86652079:_____/24:00597718 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S112578652400078X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S112578652400078X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dendro.2024.126241" target="_blank" >10.1016/j.dendro.2024.126241</a>
Alternative languages
Result language
angličtina
Original language name
Review of embedding and non-embedding techniques for quantitative wood anatomy
Original language description
In recent decades, xylem anatomical traits have become increasingly important in dendrochronological research, as they offer the unique opportunity to assess eco-physiological drivers of tree growth at intra-annual resolution. However, standard protocols for generating such data are still missing, leading to methodological uncertainty, and complicating data exchange among laboratories. Here, we compare protocols for high-quality permanent slide preparation in dendroanatomy and address the effects of paraffin embedding vs. non-embedding approaches. Tests are conducted on both gymnosperm and angiosperm wood types of widely distributed European tree species, considering cell wall thickness (CWT), mean lumen area (MLA), and hydraulic diameter (Dh). Results indicate that non-embedding does not significantly alter the qualitative and quantitative characteristics of permanent slides compared to embedded samples. Whereas the mean chronologies of MLA and Dh and their non-embedded counterparts share substantial high-frequency variance, the CWT chronologies reveal slightly larger discrepancies at inter-annual scale. However, methodological differences do not exceed 11.1 % for any parameter. While these results show high similarity between the two approaches, we recommend adopting the non-embedding procedure, since it saves resources and therefore allows to produce larger datasets. Regardless of the protocol used to build wood anatomical datasets, assembling large-scale networks of wood anatomical data could transform our understanding of forest responses to global changes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40102 - Forestry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Dendrochronologia
ISSN
1125-7865
e-ISSN
1612-0051
Volume of the periodical
88
Issue of the periodical within the volume
DEC
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
126241
UT code for WoS article
001283748900001
EID of the result in the Scopus database
2-s2.0-85199504993