Quantitative vessel mapping on increment cores: a critical comparison of image acquisition methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F25%3A00617877" target="_blank" >RIV/86652079:_____/25:00617877 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43410/25:43926747

Result on the web

<a href="https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1502237/full" target="_blank" >https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1502237/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2025.1502237" target="_blank" >10.3389/fpls.2025.1502237</a>

Result language

angličtina

Original language name

Quantitative vessel mapping on increment cores: a critical comparison of image acquisition methods

Original language description

Introduction Quantitative wood anatomy is critical for establishing climate reconstruction proxies, understanding tree hydraulics, and quantifying carbon allocation. Its accuracy depends upon the image acquisition methods, which allows for the identification of the number and dimensions of vessels, fibres, and tracheids within a tree ring. Angiosperm wood is analysed with a variety of different image acquisition methods, including surface pictures, wood anatomical micro-sections, or X-ray computed micro-tomography. Despite known advantages and disadvantages, the quantitative impact of method selection on wood anatomical parameters is not well understood.Methods In this study, we present a systematic uncertainty analysis of the impact of the image acquisition method on commonly used anatomical parameters. We analysed four wood samples, representing a range of wood porosity, using surface pictures, micro-CT scans, and wood anatomical micro-sections. Inter-annual patterns were analysed and compared between methods from the five most frequently used parameters, namely mean lumen area (MLA), vessel density (VD), number of vessels (VN), mean hydraulic diameter (D h), and relative conductive area (RCA). A novel sectorial approach was applied on the wood samples to obtain intra-annual profiles of the lumen area (A l), specific theoretical hydraulic conductivity (K s), and wood density (rho).Results Our quantitative vessel mapping revealed that values obtained for hydraulic wood anatomical parameters are comparable across different methods, supporting the use of easily applicable surface picture methods for ring-porous and specific diffuse-porous tree species. While intra-annual variability is well captured by the different methods across species, wood density (rho) is overestimated due to the lack of fibre lumen area detection.Discussion Our study highlights the potential and limitations of different image acquisition methods for extracting wood anatomical parameters. Moreover, we present a standardized workflow for assessing radial tree ring profiles. These findings encourage the compilation of all studies using wood anatomical parameters and further research to refine these methods, ultimately enhancing the accuracy, replication, and spatial representation of wood anatomical studies.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10511 - Environmental sciences (social aspects to be 5.7)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Volume of the periodical

16

Issue of the periodical within the volume

FEB

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

1502237

UT code for WoS article

001431840400001

EID of the result in the Scopus database

2-s2.0-85218880708