Phenotyping-based spectral signatures uncover barley cultivars’ sensitivity nto combined mildew and drought treatment
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F25%3A00636860" target="_blank" >RIV/86652079:_____/25:00636860 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S2772375525002333" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2772375525002333</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.atech.2025.101000" target="_blank" >10.1016/j.atech.2025.101000</a>
Alternative languages
Result language
angličtina
Original language name
Phenotyping-based spectral signatures uncover barley cultivars’ sensitivity nto combined mildew and drought treatment
Original language description
The plant’s phenotype changes under biotic and abiotic stress, reflecting its adaptations in gene expression and metabolism. For crop management, rapid detection of plant stress responses is crucial. To facilitate rapid detection of stress responses in crops, we explored the potential of UCPH’s PhenoLab for assessing barley disease resistance under both biotic and abiotic stress. We used this high-throughput macroscopic phenotyping platform to assess barley disease resistance and combined pathogen and abiotic stress response nondestructively by reflectance and fluorescence imaging over time and validate them spectroscopically in leaf extracts. At specific nwavelengths, PhenoLab spectral signatures clearly distinguished cultivars with different levels of susceptibility to the obligate biotroph pathogen Blumeria graminis (powdery mildew). Microscope phenotyping at similar reflectance and fluorescence settings parallelled the PhenoLab-derived spectral signatures. However, a specific systemic resistance response emerged three days after inoculation, detectable only by microscopy when targeting ninfected and non-infected leaf areas. We hypothesized that combined stresses would work additively and used phenotyping to study the response of the resistant and susceptible barley cultivar to a combination of drought with powdery mildew infection. Surprisingly, drought made the resistant cultivar less resistant and the susceptible one less susceptible according to changes in reflectance and fluorescence at defined wavelengths. The spectroscopic absorbance assay confirmed this result biochemically. This proof-of-concept study showcases the potential of holistic functional phenomics, using non-invasive imaging to identify predictive spectral signatures nfor barley pathogen resistance.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40101 - Agriculture
Result continuities
Project
<a href="/en/project/LM2023048" target="_blank" >LM2023048: Czech Carbon Observation System</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Smart Agricultural Technology
ISSN
2772-3755
e-ISSN
2772-3755
Volume of the periodical
11
Issue of the periodical within the volume
AUG
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
101000
UT code for WoS article
001499846300003
EID of the result in the Scopus database
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