Non-invasive nanoscale imaging of protein micro- and nanocrystals for screening crystallization conditions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2FCZ______%3A_____%2F24%3AN0000086" target="_blank" >RIV/CZ______:_____/24:N0000086 - isvavai.cz</a>

Result on the web

<a href="https://journals.iucr.org/j/issues/2024/06/00/nb5386/index.html" target="_blank" >https://journals.iucr.org/j/issues/2024/06/00/nb5386/index.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S1600576724010124" target="_blank" >10.1107/S1600576724010124</a>

Result language

angličtina

Original language name

Non-invasive nanoscale imaging of protein micro- and nanocrystals for screening crystallization conditions

Original language description

Crystallography has been the routine technique for studying high-resolution structures of proteins for over five decades. A major bottleneck in structure determination of macromolecules is obtaining crystals of a size and quality suitable for single-crystal X-ray crystallography experiments. Many challenging proteins either fail to grow into crystals or fail to grow into crystals of a size suitable for obtaining high-resolution structures using conventional X-ray crystallography. When it comes to smaller crystals, they can be used either for seeding to get larger crystals or for serial crystallography and electron diffraction for obtaining the structures. For both purposes, a limiting step is to non-invasively image such small crystals of sub-micrometre dimensions and to screen the conditions where such crystals prevail. Here we use cathodoluminescence-based (CL-based) nanoscopy to image protein nanocrystals. We show that crystals of micrometre and submicrometre dimensions can be non-invasively imaged by the CL-based nanoscope. The results presented here demonstrate the feasibility of non-invasive imaging of protein crystals with sub-100 nm resolution.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10610 - Biophysics

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF APPLIED CRYSTALLOGRAPHY

ISSN

1600-5767

e-ISSN

—

Volume of the periodical

57

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

1907-1912

UT code for WoS article

001387959000019

EID of the result in the Scopus database

2-s2.0-85210928789