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Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus
such as ELISA, one step RT-PCR and IC-PCR. Among these methods, DAS-ELISA and one step RT-PCR wasable to detect in leaves and failed to detect in corms or cormlets whereas IC-RT...
GF - Choroby, škůdci, plevely a ochrana rostlin
- 2009 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Possibility of Bean yellow mosaic virus detection in gladiolus plants by different methods
tested for the presence of BYMV using either DAS-ELISA, one step RT-PCR, real time (rt)-RT-PCR or IC-rt-RT-PCR. DAS-ELISA and one step RT-PCR were abl...
GF - Choroby, škůdci, plevely a ochrana rostlin
- 2011 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Simplyfied protocol of sample preparation for PCR and RT-PCR
Simple homogenization of sample in water, TE or TNE was sufficient for detection of some viruses and nematodes. IC-RT-PCR should be used if prezence of some inhibitors of PCR material is supposed....
GF - Choroby, škůdci, plevely a ochrana rostlin
- 2000 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Detection of Beet yellows virus by RT-PCR and immunocapture RT-PCR in Tetragonia expansa and Beta vulgaris.
with the same or higher sensitivity of subsequent PCR. Using the Triton X-100 release of RNA and IC-RT-PCR the sensitivity of detection was so high that pg amounts of BYV RNATwo sensitive methods, RT-...
GF - Choroby, škůdci, plevely a ochrana rostlin
- 2004 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
The methodical procedure for the detection of Potato spindle tuber viroid (PSTVd) by means of the techniques of Real-time RT-PCR and RT-PCR
The introduction of molecular methods (Real-time RT-PCR and RT-PCR) for the detection of Potato spindle tuber viroid (mainly in breeding materials Solanum tuberosum)....
GF - Choroby, škůdci, plevely a ochrana rostlin
- 2011 •
- NmetC
Rok uplatnění
NmetC - Metodiky certifikované oprávněným orgánem
The use of molecular techniques RT-PCR and QRT-PCR for the detection and identification of Potato Leafroll Virus (PLRV) isolates
Fifty-five isolates of PLRV from in-vitro collection (PRI Havlíčkův Brod) were tested by means of RT-PCR and QRT-PCR. The results of QRT-PCR and RT-PCR were compared with ELISA....
GF - Choroby, škůdci, plevely a ochrana rostlin
- 2015 •
- O
Rok uplatnění
O - Ostatní výsledky
Comparison of the efficiency of real-time RT-PCR and RT-PCR on field isolates of causative agent of viral haemorrhagic septicaemia from the Czech Republic
In our project we introduced the real-time RT-PCR method for detection of viral haemorrhagic septicaemia virus to our laboratory. We tested the sensitivity of the method necrosis virus. Real-time RT-PCR properties ...
GJ - Choroby a škůdci zvířat, veterinární medicina
- 2015 •
- D
Rok uplatnění
D - Stať ve sborníku
Different expression of housekeeping genes detected by quantitative real-time RT-PCR.
Choice of control gene for quantitative RT-PCR is very important for estimation of gene expression by real-time RT-PCR....
FD - Onkologie a hematologie
- 2003 •
- D
Rok uplatnění
D - Stať ve sborníku
The reaction mixture for the molecular detection of Potato spindle tuber viroid by means of quantitative RT-PCR
We designed the reaction mixture for the diagnosis of Potato spindle tuber viroid by means of quantitative RT-PCR. Potato spindle tuber viroid is a serious quarantine viroid disesase of potatoes that is possible to detect by means o...
EE - Mikrobiologie, virologie
- 2012 •
- Fuzit
Rok uplatnění
Fuzit - Užitný vzor
Real time RT PCR detection of Newcastle disease virus
DNA fragment is amplified by RT PCR Newcastle disease virus specific primers if its RNA is presented in sample. Quantity of amplified fragment is measured with TaqMan hybridization probes during RT PCR reaction. Gr...
EE - Mikrobiologie, virologie
- 2006 •
- X
Rok uplatnění
X - Nezařazeno
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