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Capillary isoelectric focusing-The role of markers of isoelectric point and recent applications in the field
Capillary isoelectric focusing (cIEF) is a very powerful separation technique. However, successful implementation of this technique in various fields of science requires a few prerequisites including a repeatable formation of pH gradient and a set of...
Analytical chemistry
- 2023 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
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Novel chip-based isoelectric focusing device for fractionation of bacteria prior to their mass spectrometry identification
We have developed a planar chip utilizing divergent geometry of separation channel capable of vertical free-flow electrophoresis of particles at flows of lower hundreds of microliters per minute. The divergent flow isoelectric focusing (DF-IEF) chip ...
Analytical chemistry
- 2022 •
- Jimp •
- Link
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Jimp - Článek v periodiku v databázi Web of Science
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Low-molecular-mass colored compounds for fine tracing of pH gradient on broad and narrow scale in isoelectric focusing
72 novel low-molecular-mass colored compounds were developed for pH gradient tracing and characterized by capillary IEF. They cover pH gradient in pH region from 2.5 to 11....
Analytical chemistry
- 2022 •
- Jimp •
- Link
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Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Development of divergent-flow isoelectric focusing chip for separation of biological agents
Isoelectric focusing (IEF) is an efficient method for separation of different bacterium species. Divergent flow format of IEF (DF-IEF) enables continual separation of large volumes of samples and fractionation of the separated analytes for later anal...
Analytical chemistry
- 2022 •
- D •
- Link
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D - Stať ve sborníku
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Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications
The host cells of S. aureus cells were dynamically adhered on the nano-structured part of FS capillary from the high conductivity matrix such as blood. Host specific bacteriophages were on-line propagated on these cells and optimized tITP/MEKC was us...
Analytical chemistry
- 2021 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Pressurized water extraction – the fast and efficient method forisolation of bioactive proteins from Viscum album leaves
In this study, utilization of the pressurized water extraction (PWE) at low temperature (40°C) for isolation of mistletoe proteins was investigated. PWE effectiveness, based on protein fingerprints, were compared with those obtained by conventional e...
Analytical chemistry
- 2021 •
- Jimp •
- Link
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Jimp - Článek v periodiku v databázi Web of Science
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Preparative continuous flow electrophoretic instrumentation for purification of biological samples
We constructed a preparative instrumentation and developed the methods that are based on separation of the samples by bidirectional isotachophoresis/moving boundary electrophoresis in continuous divergent flow. The described instrumentation can be us...
Analytical chemistry
- 2021 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Divergent flow isoelectric focusing chip for separation of bioloical agens
Functional sample is presented by prototype of chip-based device for vertical divergent flow isoelectric focusing (chip-IEF). The main part of the chip is vertically oriented separation space filled with mix of carrier ampholytes in which pH gradien...
Analytical chemistry
- 2021 •
- Gfunk •
- Link
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Gfunk - Funkční vzorek
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Separace VRA/RA ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF) pro potřeby kontroly zákazu biologických zbraní
Metodika popisuje způsob separace bakteriálních vysoce rizikových a rizikových agens (VRA/RA) ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF). Metoda chip-IEF využívá nově vyvinutý unikátní prototyp zařízení pro vertikální isoele...
Analytical chemistry
- 2021 •
- NmetC •
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NmetC - Metodiky certifikované oprávněným orgánem
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Divergent flow isoelectric focusing chip for separation of bioloical agens
Functional sample is presented by prototype of chip-based device for vertical divergent flow isoelectric focusing (chip-IEF). The main part of the chip is vertically oriented separation space filled with mix of carrier ampholytes in which pH gradient...
Analytical chemistry
- 2021 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
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