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40 202 (0,326s)

Result

How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles?

inexpensive Methylation Sensitive Amplified Polymorphism (MSAP) method and HpaII isoschizomers are used because these enzymes are methylation-sensitive, and any C within the CCGG recognition moti...

BO - Biofyzika

  • 2014
  • Jx
  • Link
Result

Assessment of epigenetic methylation changes in hop (Humulus lupulus) plants obtained by meristem culture

methylation level by methylation-sensitive amplification polymorphism (MSAP). In vitro clearly and highly reproducible amplified products were detected in the MSAP analyses within the st...

Agricultural biotechnology and food biotechnology

  • 2020
  • Jimp
  • Link
Result

Variation of cytosine methylation patterns in European beech (Fagus sylvatica L.)

as well. In this study, methylation-sensitive amplification polymorphism (MSAP of cytosine-methylation patterns at five loci and overall DNA methylation with climatic, suggesting that

Forestry

  • 2017
  • Jimp
  • Link
Result

DNA-methylation changes in grapevine somaclones following in vitro culture and thermotherapy

The methylation-sensitive amplified polymorphism (MSAP) technique using HpaII and MspI isoschizomers was used to analyse DNA-methylation alterations in stressed arrays of DNA methylation in their ...

EB - Genetika a molekulární biologie

  • 2010
  • Jx
Result

Evaluation of methylation epigenetics changes in micropropagated hop plants

by molecular methods. We assessed DNA methylation levels and variability of Saaz Osvald’s clones, ‘Sládek’ and ‘Premiant’ cultivars by methylation-sensitive amplification polymorphism (MSAP). In ...

Agricultural biotechnology and food biotechnology

  • 2021
  • D
  • Link
Result

Does DNA methylation pattern mark generative development in winter rape?

. The methylation-sensitive amplification polymorphism (MSAP) method with EcoRI/MspI and EcoRIIIn this paper we report on changes in DNA methylation pattern in rape apices with vernalized non-graf...

EF - Botanika

  • 2006
  • Jx
Result

DNA Methylation and Transcriptomic Changes in Response to Different Lights and Stresses in 7B-1 Male-Sterile Tomato

-specific resistance to abiotic and biotic stresses. Using a methylation-sensitive amplifiedpolymorphism (MSAP) assay, a number of genes were identified, which were differentially methylated between 7B-1 and its wi...

EB - Genetika a molekulární biologie

  • 2015
  • Jx
  • Link
Result

Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.)

-retrotransposon amplified polymorphism (IRAP) method. However, the more global amplified fragment length polymorphism (AFLP) and the methylation sensitive MSAP methods detected 11 and 18% polymorphism...

EB - Genetika a molekulární biologie

  • 2007
  • Jx
Result

Evaluation of the AFLP and MSAP methods as tools for studying of DNA changes in grapevine plants long period after their in vitro thermotherapy

structure of DNA, AFLP (Amplified Fragment Length Polymorphism) was applied in this study. Epigenetic changes were determined by means of MSAP (Methylation Sensitive Amplified Polymorphism). Studies were ...

GE - Šlechtění rostlin

  • 2012
  • Jx
Result

Comparison of DNA methylation landscape between Czech and Armenian vineyards show their unique character and increased diversity

. Results of the Methylation Sensitive Amplified Polymorphism method confirm uniqueness theoretically be driven by individual vineyards' conditions represents DNA methylation. The aim of this work is to examine whe...

Agriculture

  • 2021
  • Jimp
  • Link
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