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Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery
considerations that have to be taken into account when using the yeast expression system), and expression vector choice; and provide detailed protocols for yeast transformationThe budding yeast (Saccharom...
EB - Genetika a molekulární biologie
- 2014 •
- C •
- Link
Rok uplatnění
C - Kapitola v odborné knize
Výsledek na webu
Heterologous expression of mammalian Na/H antiporters in Saccharomyces cerevisiae
Heterologous expression of mammalian Na/H antiporters in a mutant strain Saccharomyces cerevisiae lacking its own systems for alkali metal cation efflux showed the suitability of yeast as a model and revealed new properties...
EB - Genetika a molekulární biologie
- 2006 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Vector encoding gene for expression of RNA helicase in E. coli, originating from linear plasmids of the yeast Kluyveromyces lactis
An expression vector carrying gene for RNA helicase encoded by linear plasmids of Kluyveromyces lactis (ORF4-pGKL2) was prepared. The sequence encoding RNA helicase was optimized for heterologous expression in E.coli. RNA h...
Biological sciences
- 2024 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Vector encoding gene for expression of DNA polymerase enzyme in E. coli, originating from linear plasmids of the yeast Kluyveromyces lactis
An expression vector carrying gene for DNA polymerase encoded by linear plasmids of Kluyveromyces lactis (ORF2-pGKL2) was prepared. The sequence encoding DNA polymerase was optimized for heterologous expression in E.coli. D...
Biological sciences
- 2024 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Vector encoding gene for expression of RNA capping enzyme in E. coli, originating from linear plasmids of the yeast Kluyveromyces lactis
An expression vector carrying gene for RNA capping enzyme encoded by linear plasmids of Kluyveromyces lactis (ORF3-pGKL2) was prepared. The sequence encoding RNA capping enzyme was optimized for heterologous expression in E...
Biological sciences
- 2024 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Mammalian NHE2 Na(+)/H(+) exchanger mediates efflux of potassium upon heterologous expression in yeast
Mammalian NHE2 exchanger (antiporter) was expressed in a Saccharomyces cerevisiae mutant strain lacking its own exporters of alkali metal cations and mediated K+ efflux from the yeast. We demonstrate for the first time that a mammal...
ED - Fyziologie
- 2005 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Set of vectors encoding genes for expression of RNA polymerase in E. coli, originating from linear plasmids of the yeast Kluyveromyces lactis
A set of 2 vectors carrying genes for RNA polymerase encoded by linear plasmids of Kluyveromyces lactis (ORF6-pGKL2 and ORF7-pGKL2) was prepared. The sequences encoding RNA polymerase were optimized for heterologous expression in E....
Biological sciences
- 2024 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Production of polyoma virus capsid protein VP1 in yeast Saccharomyces cerevisiae under different inducible promoters
XXth International Conference on Yeast Genetics and Molecular Biology...
EE - Mikrobiologie, virologie
- 2001 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata
(for expression of Ena1 proteins tagged with GFP at their C termini). Resulting plasmids allow the heterologous expression of Candida glabrata Ena1 transporter in S. cerevisiae strains......
EE - Mikrobiologie, virologie
- 2009 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Set of multicopy vectors for the expression of genes in Zygosaccharomyces rouxii
A set of 3 multicopy Z.rouxii/E.coli shuttle vectors with different marker genes (ScURA3, ZrLEU2, ZrADE2) has been constructed for the osmotolerant yeast Z. rouxii in order to allow the overproduction of a desired protein encoded by the gene...
EE - Mikrobiologie, virologie
- 2008 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
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