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Expression vector for bacterial production of antigen streptavidin
By using methods of genetic engineering the production plasmid vector for antigen streptavidin was constructed and verified, the vector carry entire antigen sequence including C-terminal His tag. The antigen can be...
EE - Mikrobiologie, virologie
- 2007 •
- X
Rok uplatnění
X - Nezařazeno
Expression vectors for bacterial intracellular production of recombinant pol (retroviral reverse polymerasis) protein and its genetic fusion with streptavidin in E. coli.
Pol is one of the immunodominant antigen of Rous sarcoma virus. This antigen was genetically attached to streptavidin. Using genetic engineering methods we have created and verified expression vectors coding the natural
EI - Biotechnologie a bionika
- 2014 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Expression vectors for bacterial intracellular production of recombinant VP2 protein in E. coli.
. As immunogenic protein the VP2 antigen is usually described. Two variants of fusion proteins were designed and produced; the streptavidin fused with N-terminal part of VP2 antigen (including immunogenic epitope GNVLVGEGV)...
EI - Biotechnologie a bionika
- 2016 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Recombinant chimeric materials for in vitro stimulation of specific cellular immune response against hCMV
Using genetic engineering we constructed expression vectors carrying the genetic fusion of streptavidin with selected CMV-specific antigen that will be used for restimulation of whole blood or blood cells, for the detection of cytom...
EI - Biotechnologie a bionika
- 2013 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Expression vectors for bacterial intracellular production of recombinant env protein and its genetic fusion with streptavidin in E. coli
Env is one of the immunodominant antigen Rous sarcoma virus. This antigen was genetically attached to streptavidin. Due to size, env protein was divided into 2 vectors for intracellular expression of sequence of natural
EI - Biotechnologie a bionika
- 2013 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Expression vectors for bacterial intracellular production of recombinant pIVa2 protein and its genetic fusion with streptavidin in E. coli.
coding the natural streptavidin core fused with the pIVa2 antigen on its C-terminal of cellular activities. It has 380 aminoacids. It was genetically attached to streptavidin experiments a free recombinant pIVa2 p...
EI - Biotechnologie a bionika
- 2015 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Expression vectors for bacterial intracellular production of recombinant HexonIIIa protein and its genetic fusion with streptavidin in E. coli.
the natural streptavidin core fused with the HexonIIIa antigens on its C-terminal end-290; 250-539). These parts were genetically attached to streptavidin. Using recombinant HexonIIIa protein parts were prepared....
EI - Biotechnologie a bionika
- 2014 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Expression vectors for bacterial intracellular production of recombinant hexon protein and its genetic fusion with streptavidin in E. coli.
genetically attached to streptavidin. Using genetic engineering methods we have created and verified expression vectors coding the natural streptavidin core fused with the Hexon antigens on its C-terminal end. The resultin...
EI - Biotechnologie a bionika
- 2014 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Expression vectors for bacterial intracellular production of recombinant gag (retroviral capsid protein) protein and its genetic fusion with streptavidin in E. coli.
Streptavidin in its tetrameric form is widely used in bio-analytical applications, mainly due to its high affinity for biotin. By using methods of genetic engineering the expression plasmid vectors for intracellular expression of sequence of...
EI - Biotechnologie a bionika
- 2014 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Complexes of Streptavidin-Fused Antigens with Biotinylated Antibodies Targeting Receptors on Dendritic Cell Surface: A Novel Tool for Induction of Specific T-Cell Immune Responses
The choice of tools that enable efficient targeting of exogenous antigens (Ag) for processing and presentation by professional Ag-presenting cells (APC) remains limited. This represents, indeed, a bottleneck in development of vaccines induci...
EE - Mikrobiologie, virologie
- 2012 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
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