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Analysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence
In many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach an extraordinary sensitivity of analyses. Semiconductor nanoparticles, quantum dots, have already pr...
CB - Analytická chemie, separace
- 2014 •
- C •
- Link
Rok uplatnění
C - Kapitola v odborné knize
Výsledek na webu
Recent advances in the development of single cell analysis-A review
Development of techniques for the analysis of the content of individual cells represents an important direction in modern bioanalytical chemistry. While the analysis of chromosomes, organelles, or location of selected proteins has been traditionally ...
CB - Analytická chemie, separace
- 2013 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptid...
CB - Analytická chemie, separace
- 2013 •
- D
Rok uplatnění
D - Stať ve sborníku
Recent advances in the combination of capillary electrophoresis with mass spectrometry: From element to single-cell analysis
This overview deals with the latest development of electrophoresis in capillaries and microfluidic devices coupled to MS detection. A wide selection of relevant articles covers the literature published from January 2010 till June 2012 as a continuati...
CB - Analytická chemie, separace
- 2013 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
Bioluminescence determination of active caspase-3 in single apoptotic cells
In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. The technology is based on the specific cleavage of modified luciferin by caspase-...
CB - Analytická chemie, separace
- 2013 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
Senzitive method of Caspase-3 detection in single stem cell
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/l...
EB - Genetika a molekulární biologie
- 2013 •
- D
Rok uplatnění
D - Stať ve sborníku
Analysis of tissue-bound cells: Novel approaches using laser capture microdissection
Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as signalling centres in embryonic development, stem cell...
CB - Analytická chemie, separace
- 2013 •
- D
Rok uplatnění
D - Stať ve sborníku
Dynamics of caspase-3 activation and inhibition in embryonic micromasses evaluated by a photon-counting chemiluminiscence approach
Caspases are key enzymatic components of the intracellular apoptotic machinery and their role in mammalian systems is often studied using fluoromethylketone (FMK) inhibitors. This work therefore focuses on an exact evaluation of caspase-3 FMK inhibit...
ED - Fyziologie
- 2012 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
A simple device for quantitation of Caspase 3 in individual apoptotic embryonic cells
A newly developed system for the determination of Caspase 3 activity in individual apoptotic embryonic cells is described. Caspase 3, a cysteine-aspartic acid protease activated in the apoptotic pathway, plays an essential role in the programmed cell...
CB - Analytická chemie, separace
- 2012 •
- D •
- Link
Rok uplatnění
D - Stať ve sborníku
Výsledek na webu
Simultaneous analysis of cocaine and its metabolites in urine by capillary electrophoresis - electrospray mass spectrometry using a pressurized liquid junction nanoflow interface
A new method for the simultaneous separation of cocaine and four metabolites in urine by CE-ESI-MS via a pressurized nano-liquid junction interface was developed. The LODs achieved with the field amplified sample injection method ranged from 1.5 to 1...
CB - Analytická chemie, separace
- 2012 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
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