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Enzymatic removal of N-glycans by PNGase F coated magnetic microparticles
In this paper, we report on the use of PNGase F immobilization onto the surface ofmagnetic microparticles and their use in rapid and efficient removal of N-glycans from glycoproteins....
CB - Analytická chemie, separace
- 2016 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release
We demonstrate a simple and rapid method for the oriented immobilization of peptide-N4-(Nacetyl- glucosaminyl) asparagine amidase F (PNGase F) on a porous polymer-transferase(GST) tagged PNGase F towards g...
CB - Analytická chemie, separace
- 2012 •
- D •
- Link
Rok uplatnění
D - Stať ve sborníku
Výsledek na webu
Oriented immobilization of peptide-N-glycosidase F on a monolithic support for glycosylation analysis
In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid, reproducible and efficient release of the N-linked carbohydrate m...
CB - Analytická chemie, separace
- 2013 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
Thiol-ene-based monolithic enzymatic microreactor for glycoprotein deglycosylation
Enzymatic microreactor was fabricated from thiol-ene polymer. Glycoprotein samples were deglycosylated by enzyme PNGase F which was immobilized on the monolith....
CB - Analytická chemie, separace
- 2015 •
- D •
- Link
Rok uplatnění
D - Stať ve sborníku
Výsledek na webu
Development of a PNGase Rc Column for Online Deglycosylation of Complex Glycoproteins during HDX-MS
developed a column containing immobilized PNGase Rc (from Rudaea cellulosilytica analysis of glycoproteins. We show that HDX-MS with the PNGase Rc column enables of glycosylated regions in several complex glycoproteins. Additionall...
Biochemistry and molecular biology
- 2023 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors
F (PNGase F) were immobilized at the surface of the synthesized thiol-ene...
CB - Analytická chemie, separace
- 2015 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
Efficient Procedure for N-Glycan Analyses and Detection of Endo H-Like Activity in Human Tumor Specimens
or absence of water prior to PNGase F deglycosylation. The procedure was successfully...
CB - Analytická chemie, separace
- 2016 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity
-TBN1 digested by alpha-mannosidase, and R-TBN1 deglycosylated by PNGase F were...
Biochemistry and molecular biology
- 2018 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Efficient Procedure for N-Glycan Analyses and Detection of Endo H-Like Activity in Human Tumor Specimens
or absence of water prior to PNGase F deglycosylation. The procedure was successfully...
Clinical medicine
- 2016 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Testing the adherence of commensal and pathogenic bacteria to monolayers of human intestinal epithelial cells as means for selection of bacteria-vectored recombinant vaccines
-IgA was incubated with PNGase F enzyme which cleaves Nlinked glycans...
Immunology
- 2018 •
- D
Rok uplatnění
D - Stať ve sborníku
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