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10 154 (0,111s)

Result

Identification of chromosomal fusion sites in Arabidopsis mutants using sequential bicolour BAC-FISH

The method of sequential bicolour BAC-FISH enables unequivocal assignment of chromosome/chromatid fusion sites. The particular types of chromosome fusions in Arabidopsis thaliana model plant defficient in telomerase gene are shown....

BO - Biofyzika

  • 2006
  • Jx
Result

New methods of physical mapping of plant genomes.

We present several modern techniques of physical mapping of plant genomes including (i) construction and the use of BAC libraries, (ii) fluorescence in situ hybridization (FISH) with BAC clones and (iii) localization of gen...

BO - Biofyzika

  • 2003
  • Jx
Result

Conserved synteny of genes between chromosome 15 of Bombyx mori and a chromosome of Manduca sexta shown by five-color BAC-FISH

of BAC-FISH. This technique was established in Bombyx mori for two differently labeled BAC probes simultaneously hybridized to pachytene bivalents. In order to achieve higher-throughput comparative mapping using BAC

EB - Genetika a molekulární biologie

  • 2007
  • Jx
Result

FISH analysis of the W chromosome in Bombyx mandarina and several other species of Lepidoptera by means of B. mori W-BAC probes

-BAC) clones from Bombyx mori BAC libraries by means of six different STS primer sets. Two colour FISH with 19L6H W-BAC probe as a standard W-chromosome painter disclosed thatsix out of seven new W-BAC pro...

EB - Genetika a molekulární biologie

  • 2007
  • Jx
Result

Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping

then constructed a BAC-FISH karyotype identifying all 28 chromosomes of M. sexta by mapping 124We used fluorescence in situ hybridization (FISH) with bacterial artificial chromosome (BAC) probes for physical mappi...

EB - Genetika a molekulární biologie

  • 2009
  • Jx
Result

Structure and evolution of plant sex chromosomes: The use of BAC library.

Sex chromosomes of plant species Silene latifolia are the ideal model for evolutionary biology studies because they represent early stages of X and Y chromosomes history. We used fluorescence in situ hybridization (FISH) with bacterial artif...

BO - Biofyzika

  • 2002
  • D
Result

Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones

Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms often, contigs of chromosome-specific bacterial artificial chromosomes (...

EB - Genetika a molekulární biologie

  • 2016
  • Jx
  • Link
Result

Localization of BAC clones on mitotic chromosomes of Musa acuminata using fluorescence in situ hybridization

A bacterial artificial chromosome (BAC) library of banana (Musa acuminata) was used to select BAC clones that carry low amounts of repetitive DNA sequences and could be suitable as probes for fluorescence in situ hybridization (...

EF - Botanika

  • 2008
  • Jx
Result

Construction and characterization of a BAC library for functional genomics in Xenopus tropicalis

localization using FISH and by systematic BAC end sequencing. The median insert size such as BAC libraries are lagging behind. Here we present the construction and characterization of a BAC library that covers the...

Biological sciences

  • 2017
  • Jimp
  • Link
Result

Fluorescent in situ Hybridization shows DIPLOSPOROUS located on one of the NOR chromosomes in apomictic dandelions in the absence of a large hemizygous chromosomal region

on the nucleolus organizer region (NOR) chromosomes that contain the 45SrDNAs. FISH with S4-BAC in Taraxacum by using fluorescent in situ hybridization (FISH) with bacterial artificial chromosomes (BACs) that gene...

EF - Botanika

  • 2014
  • Jx
  • Link
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