TPR, chromatin organization, gene expression and cell development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F19%3A10400201" target="_blank" >RIV/00216208:11130/19:10400201 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=rqc4A.vyG_" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=rqc4A.vyG_</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7124/bc.0009A6" target="_blank" >10.7124/bc.0009A6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

TPR, chromatin organization, gene expression and cell development

Popis výsledku v původním jazyce

The area around nuclear pores is enriched in open chromatin. Transcriptional factors and super enhancer sequences localize there during cell differentiation, activating sets of genes specific for a given tissue. Nucleoporin TPR is crucial for the enrichment of open chromatin around nuclear pores, however its role in gene expression and cell development has not been described yet. Here we show that depletion of TPR results in aberrant morphology of murine proliferating C2C12 myoblasts (MB) and differentiated C2C12 myotubes (MT). Our CHIP-seq data revealed that TPR binds to Myosin heavy chain (Myh) gene and majority of olfactory receptor (Olfr) genes in C2C12 MB, and its binding pattern changes upon differentiation into MT. Both Myh and Olfr are expressed in muscle cell and regulate the muscle formation and morphology. We show that TPR affects expression of both Myh and Olfr genes, however in a different manner. Here we discuss possible pathways by which TPR regulates expression of bound genes. This work was supported by the GACR (15-08835Y, 16-03403S, 18-19714S), GAUK (930218) and by the institutional support (RVO: 68378050). I acknowledge the Microscopy Centre funded by MEYS CR (LM2015062, Czech-BioImaging) for their support. (C) 2019, National Academy of Sciences of Ukraine. All rights reserved.

Název v anglickém jazyce

TPR, chromatin organization, gene expression and cell development

Popis výsledku anglicky

The area around nuclear pores is enriched in open chromatin. Transcriptional factors and super enhancer sequences localize there during cell differentiation, activating sets of genes specific for a given tissue. Nucleoporin TPR is crucial for the enrichment of open chromatin around nuclear pores, however its role in gene expression and cell development has not been described yet. Here we show that depletion of TPR results in aberrant morphology of murine proliferating C2C12 myoblasts (MB) and differentiated C2C12 myotubes (MT). Our CHIP-seq data revealed that TPR binds to Myosin heavy chain (Myh) gene and majority of olfactory receptor (Olfr) genes in C2C12 MB, and its binding pattern changes upon differentiation into MT. Both Myh and Olfr are expressed in muscle cell and regulate the muscle formation and morphology. We show that TPR affects expression of both Myh and Olfr genes, however in a different manner. Here we discuss possible pathways by which TPR regulates expression of bound genes. This work was supported by the GACR (15-08835Y, 16-03403S, 18-19714S), GAUK (930218) and by the institutional support (RVO: 68378050). I acknowledge the Microscopy Centre funded by MEYS CR (LM2015062, Czech-BioImaging) for their support. (C) 2019, National Academy of Sciences of Ukraine. All rights reserved.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biopolymers and Cell

ISSN

0233-7657

e-ISSN

—

Svazek periodika

35

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

UA - Ukrajina

Počet stran výsledku

1

Strana od-do

169

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85073394527