Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00110713" target="_blank" >RIV/00216224:14310/19:00110713 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf" target="_blank" >http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach
Popis výsledku v původním jazyce
Dental calculus has great potential in the research of oral microbiome in past populations. Alongside the advantages of novel molecular approaches applied on ancient human dental calculus, we encounter the drawbacks as well. One of the main issues is contamination. There is a possibility of detection of microbial contamination by scanning electron microscopy prior to subsequent expensive molecular analysis. Although decontamination is always part of the workflow, the metagenomic analysis points out the residual microbial contamination. This study presents the means of detecting contamination and precautions to be taken for the best prevention of it.
Název v anglickém jazyce
Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach
Popis výsledku anglicky
Dental calculus has great potential in the research of oral microbiome in past populations. Alongside the advantages of novel molecular approaches applied on ancient human dental calculus, we encounter the drawbacks as well. One of the main issues is contamination. There is a possibility of detection of microbial contamination by scanning electron microscopy prior to subsequent expensive molecular analysis. Although decontamination is always part of the workflow, the metagenomic analysis points out the residual microbial contamination. This study presents the means of detecting contamination and precautions to be taken for the best prevention of it.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů