*Inovation of real-time PCR probes
Project goals
*This project is aimed on innovation of probes for real-time PCR - a molecular-biology method that serves for precise quantification of nucleic acid sequences. This method is applied in many fields for precise quantification of gene expression, allelic discrimination and pathogen detection. Hydrolysation dual-labeled fluorescent probes are the most frequent type of probes. In many cases the probes prepared by standard procedures have a very low ratio of fluorescence between activated and non-activated state. This problem leads to a late response of real-time PCR machines (cyclers) and thus to lower sensitivity. In this project we aimed on improvement of real-time PCR probes in two ways - innovation of their design and synthesis and further the preparation of own quenchers. The innovated real-time PCR probes will be offered as new product and further they will be implemented into kits for quantification of gene expression and pathogen detection.
Keywords
Public support
Provider
Ministry of Industry and Trade
Programme
TIP
Call for proposals
TIP 1 (SMPO2009/01)
Main participants
—
Contest type
VS - Public tender
Contract ID
FR-TI1/112
Alternative language
Project name in Czech
*Inovace sond pro real-time PCR
Annotation in Czech
*Tento projekt se zaměřuje na inovaci sond pro real-time PCR - molekulárně biologické metody, která slouží k přesné kvantifikaci sekvencí nukleových kyselin. Tato metoda našla uplatnění v mnoha oborech, kde se převážně používá pro kvantifikaci exprese genů, alelickou diskriminaci a detekci patogenů. Hydrolyzační dvojitě značené fluorescenční sondy jsou nejčastěji používaným typem sond. V mnoha případech však standardně připravené sondy mají velmi nízký poměr fluorescence mezi aktivovanou (hydrolyzovanou) sondou a sondou neaktivní. To vede k pozdější odezvě u real-time PCR přístrojů a tím ke snížení citlivosti. V tomto projektu se snažíme o zvýšení kvalit real-time PCR sond ze dvou směrů - jednak inovovat design a syntézu a dále přípravou vlastních zhášečů fluorescence. Inovované sondy budou nabízeny jednak jako samostatný produkt a dále budou uplatněny v našich soupravách pro kvantifikaci genové exprese a detekce patogenů.
Scientific branches
R&D category
AP - Applied research
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
EI - Biotechnology and bionics
CEP - another secondary branch
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10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30101 - Human genetics
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
A pethod for labeling and resign of probes was developed and launched as a new service. The results were used for innovation of kits for the quantification of reference genes and in a new product - The kit for the qvantification of chlamydia trachomatis.
Solution timeline
Realization period - beginning
Jul 1, 2009
Realization period - end
Dec 31, 2012
Project status
U - Finished project
Latest support payment
Apr 20, 2012
Data delivery to CEP
Confidentiality
C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.
Data delivery code
CEP13-MPO-FR-U/02:2
Data delivery date
Jul 14, 2014
Finance
Total approved costs
15,712 thou. CZK
Public financial support
12,553 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
3,158 thou. CZK
Basic information
Recognised costs
15 712 CZK thou.
Public support
12 553 CZK thou.
79%
Provider
Ministry of Industry and Trade
CEP
EB - Genetics and molecular biology
Solution period
01. 07. 2009 - 31. 12. 2012