Impact of humanised glycosylation pathway on protein accumulation and traficking in plant seeds
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
Standardní projekty 19 (SGA0201500001)
Main participants
Agritec Plant Research s.r.o.<br>Ústav experimentální botaniky AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
15-10768S
Alternative language
Project name in Czech
Studium vlivu humanizované glycosylace na akumulaci proteinů v semenech rostlin
Annotation in Czech
V tomto projektu budeme modifikovat glykosylační dráhu v semenech rostlin tak, aby vznikající glykoproteiny odpovídaly savčím a zejména lidským glykoproteinům. Pomocí postupů syntetické biologie sestavíme vícegenový konstrukt, obsahující geny z metabolických drah lidské glykosylace a umlčující geny zodpovědné syntézu specifických a1,2-xylozových a a1,3-fukozových glykoproteinů pod kontrolou promotorů aktivních v semenech. Těmito konstrukty budou poté transformovány jak modelové rostliny (A. thaliana a N. benthamiana) tak i luštěniny sója a hrách (G. max a P. sativum). Budou zjištěny glykosylační profily modelové lidské protilátky jakož i hlavních rostlinných glykoproteinů.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
GE - Plant cultivation
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
EI - Biotechnology and bionics
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30101 - Human genetics<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40105 - Horticulture, viticulture<br>40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
The project has been focused on modification of the glycosylation pathway of plant seeds to mimic glycosylation pattern observed in mammalian and particularly in human cells, in both model (A. thaliana and N. benthamiana) and leguminous plant species (G. max and P. sativum). Main aim of this ambitious project has not been fulfilled and substantial results have not been published up till now.
Solution timeline
Realization period - beginning
Jan 1, 2015
Realization period - end
Nov 29, 2019
Project status
U - Finished project
Latest support payment
Apr 5, 2017
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP20-GA0-GA-U/01:1
Data delivery date
Jul 2, 2020
Finance
Total approved costs
7,538 thou. CZK
Public financial support
7,538 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK