Functional Genomics of Dioecious Plants
Public support
Provider
Ministry of Education, Youth and Sports
Programme
KONTAKT II
Call for proposals
KONTAKT II 4 (SMSM2014LH4)
Main participants
Biofyzikální ústav AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-9919/2014-1
Alternative language
Project name in Czech
Funkční genomika dvoudomých rostlin
Annotation in Czech
Cílem projektu je strukturní a funkční analýza pohlavně vázaných genů u modelových dvoudomých rostlin Silene latifolia a Rumex acetosa. K dosažení tohoto cíle je mj. nutné zvládnout nové technologie genetické modulace studovaných genů. Během tří let plánovaného projektu budou identifikovány geny vázané na pohlavní chromosomy, vyvinuta metodika pro cílenou mutagenezi a umlčování studovaných genů. Tento projekt by měl zásadně přispět k objasnění funkce pohlavních drah u modelových dvoudomých rostlin.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
EF - Botany
CEP - another secondary branch
EI - Biotechnology and bionics
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>10611 - Plant sciences, botany<br>10612 - Mycology<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30101 - Human genetics<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The findings accomplished in previous stages, were integrated during the last year of the project. Thanks to our recent elaboration of transformation protocol, the CRISPR/Cas9 constructs were successfully delivered to plants and we isolated mutants in AP3 gene. Furthermore we successfully tested the possibility to employ the amiRNA and gene targeting methods in S. latifolia. In last year of the project, the fourth internship in laboratory of our foreign partner was carried out. During the whole period of the project, four publications were published in high impact journals (BMC Genomics, Genome Biology a Nature Plants).
Solution timeline
Realization period - beginning
Apr 1, 2014
Realization period - end
Dec 31, 2016
Project status
U - Finished project
Latest support payment
Feb 18, 2016
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP17-MSM-LH-U/01:1
Data delivery date
Jun 23, 2017
Finance
Total approved costs
4,557 thou. CZK
Public financial support
1,780 thou. CZK
Other public sources
2,777 thou. CZK
Non public and foreign sources
0 thou. CZK