Immunological analysis of sand fly - host - Leishmania interactions
Project goals
Sand flies are the vectors of Leishmania parasites - and their saliva plays an important role in Leishmania infection development. Pre-exposure to sand fly bites can protect the host against disease development. The aim of the project is to characterizethe immune response of repeatedly bitten host in relation to Leishmania infection.We will study the influence of short- and long-term exposure to sand fly bites on Leishmania infection development. The course of infection will be monitored by measuring the lesion size and by parasite quantification by PCR-ELISA. The status of host immunity will be characterized by measuring cytokine and chemokine production, anti-saliva antibodies, and the capability of macrophages to produce NO. The specificity of hostimmune response to certain sand fly species will be tested as well. The data will help to characterize the role of sand fly saliva in Leishmania transmission and possible utilization of sand fly saliva in transmission blocking vaccine.
Keywords
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
Standardní projekty 12 (SGA02009GA-ST)
Main participants
—
Contest type
VS - Public tender
Contract ID
206/09/0822
Alternative language
Project name in Czech
Imunologie vztahů flebotomus - hostitel - leishmanie
Annotation in Czech
Na modelu myš kmene Balb/c-Phlebotomus duboscqi-Leishmania major chceme zjistit (1) vliv různě dlouhé imunizace sáním flebotomů na vývoj leishmaniové infekce a na stav imunitního systému hostitele a (2) specifitu imunitního systému imunizovaného hostitele vůči antigenům jiného druhu flebotoma.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EC - Immunology
CEP - secondary branch
EG - Zoology
CEP - another secondary branch
FN - Epidemiology, infection diseases and clinical immunology
10613 - Zoology
10614 - Behavioral sciences biology
10615 - Ornithology
10616 - Entomology
30102 - Immunology
30302 - Epidemiology
30303 - Infectious Diseases
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project was excellent, both in terms of expertise and in terms of disbursement of funds.
Solution timeline
Realization period - beginning
Jan 1, 2009
Realization period - end
Dec 31, 2011
Project status
U - Finished project
Latest support payment
Apr 16, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-GA0-GA-U/02:2
Data delivery date
May 2, 2016
Finance
Total approved costs
2,187 thou. CZK
Public financial support
2,187 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
2 187 CZK thou.
Public support
2 187 CZK thou.
100%
Provider
Czech Science Foundation
CEP
EC - Immunology
Solution period
01. 01. 2009 - 31. 12. 2011