Toolkit and principles of mitoribosome biogenesis and its interplay with RNA editing
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
SGA0202600001
Main participants
Biologické centrum AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
26-20429S
Alternative language
Project name in Czech
Toolkit and principles of mitoribosome biogenesis and its interplay with RNA editing
Annotation in Czech
High complexity and dual genetic origin of mitoribosomes, ribonucleoprotein complexes responsible for proteins synthesis in mitochondria, are reflected in their intricate assembly processes relying on multiple assembly factors. Early stages of mitoribosome biogenesis, unlike late steps, remain obscured. Here, we will utilize the protist Trypanosoma brucei to understand the early assembly of the small mitoribosomal subunit (mtSSU), which is difficult to study in other model organisms. We will combine biochemical and proteomic approaches to identify and characterize early assembly factors widespread in eukaryotes and specific to trypanosomatids. Using cryoEM, we will determine structures of early assembly intermediates to reveal the conserved and group-specific mechanisms of early biogenesis. We will also rationalize a unique direct link we found between an mtSSU precursor and U-indel RNA editing. Our results will provide grounds for development of diagnostic or therapeutic tools against mitoribosome-associated mitochondrial disorders and diseases caused by trypanosomatids parasites.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10608 - Biochemistry and molecular biology
OECD FORD - secondary branch
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OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
CE - Biochemistry<br>EB - Genetics and molecular biology
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2028
Project status
Z - Beginning multi-year project
Latest support payment
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Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GA-R
Data delivery date
May 4, 2026
Finance
Total approved costs
9,708 thou. CZK
Public financial support
9,708 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK