Distal and proximal controllers of gene activity in barley
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
SGA0202600001
Main participants
Ústav experimentální botaniky AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
26-23397S
Alternative language
Project name in Czech
Distal and proximal controllers of gene activity in barley
Annotation in Czech
Gene transcription is controlled by an interplay of transcription factors and cis-regulatory elements (CREs) – promoters, enhancers and silencers. A growing number of studies point to an association between the CREs and advantageous traits in crops, reinforcing interest in these elements. Previously, we intersected data from epigenome and interactome profiling in barley embryo and predicted over 2000 putative distal CREs and their target genes. Here, we plan to assess possible impact of these CREs on the transcription of their targets by comparing data from barley pan-genome and pan-transcriptome. Promising CREs will be validated by a luciferase reporter assay. Moreover, CREs of two developmentally regulated genes affecting agronomically important traits – GTL1 and SWEET11b - will be validated by CRISPR-Cas editing. Finally, to assess a predicted upstream regulator of SWEET11b, we will remove its binding site from the SWEET11b promoter. Results of this project will increase the knowledge of cis-regulation in plants and provide possible targets for bioengineering in cereal crops.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10603 - Genetics and heredity (medical genetics to be 3)
OECD FORD - secondary branch
10605 - Developmental biology
OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
EB - Genetics and molecular biology
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2028
Project status
Z - Beginning multi-year project
Latest support payment
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Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GA-R
Data delivery date
Apr 30, 2026
Finance
Total approved costs
6,939 thou. CZK
Public financial support
6,939 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK