Inhibitors of insect digestive enzymes associated with natural defence of plants against pests
Project goals
Plant inhibitors of insect digestive enzymes play an important role in natural defense of pants. Spectrum of both inhibitors and enzymes is changing during the complex process of plant-insect interaction (wound-induced inhibitors, inhibitor-induced enzymes) The potential of protease and alfa-amylase inhibitors to block the digestive enzymes of insects will be studied both in vivo and in vitro. The project includes isolation and characterization of selected plant inhibitors from potato, tomato and bean.The insect digestive enzymes (proteases and a-amylases) from several herbivores and stored grain pests will be analyzed. We intend to investigate biochemical specificity of inhibitors and enzymes, their interaction, and changes during the inductionprocess in plants and insects. The effects of ingested inhibitors on the insects will be described in terms of biological parameters (e.g. growth rate, mortality, fecundity). The inhibitors will be supplied in the diet, or their levels will be modulated
Keywords
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
Standardní projekty 1 (SGA02002GA-ST)
Main participants
Ústav organické chemie a biochemie AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
—
Alternative language
Project name in Czech
Úloha inhibitorů trávících enzymů hmyzu v obranyschopnosti rostlin vůči škůdcům
Annotation in Czech
V přirozené obranyschopnosti rostlin proti škůdcům hrají významnou úlohu rostlinné inhibitory trávicích enzymů hmyzu. Spektrum inhibitorů i enzymů se mění během komplexního procesu interakce rostliny s hmyzem (inhibitory indukované poraněním rostliny, enzymy indukované přítomností inhibitorů). Proteázové a a-amylázové inhibitory izolované z tkání bramboru, rajčete a fazole budou biochemicky charakterizovány a bude studován jejich inhibiční potenciál v testech in vivo a in vitro. Hmyzí trávicí enzymy (proteázy a a-amylázy) budou analyzovány u vybraných druhů herbivorů a skladištních škůdců. Výzkum bude zaměřen na biochemickou specificitu inhibitorů a enzymů, jejich interakci, a změny jejich složení během indukčních procesů u rostliny a hmyzu. Účinky inhibitorů požitých hmyzem budou sledovány na biologických parametrech (např. rychlost růstu, mortalita, plodnost). Hladiny inhibitorů v potravě hmyzu budou řízeny obohacením potravy isolovanými inhibitory nebo ošetřením živných rostlin chemickými
Scientific branches
R&D category
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CEP classification - main branch
GF - Diseases, pests, weeds and plant protection
CEP - secondary branch
EI - Biotechnology and bionics
CEP - another secondary branch
CE - Biochemistry
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
Projekt byl velmi dobře koncipován. Výsledky byly převážně prezentovány na konferencích, práce o inhibitorech alfa-amyláz je připravena do tisku ve vědeckém časopise. Tématicky projektu odpovídá práce publikovaná v J. Chem. Ecol., byla však použita jiná
Solution timeline
Realization period - beginning
Jan 1, 2000
Realization period - end
Jan 1, 2002
Project status
U - Finished project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP/2003/GA0/GA03GA/U/N/9:7
Data delivery date
May 19, 2008
Finance
Total approved costs
2,745 thou. CZK
Public financial support
2,031 thou. CZK
Other public sources
968 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
2 745 CZK thou.
Public support
2 031 CZK thou.
73%
Provider
Czech Science Foundation
CEP
GF - Diseases, pests, weeds and plant protection
Solution period
01. 01. 2000 - 01. 01. 2002