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Insecticidal proteins targeted against insect midgut

Public support

  • Provider

    Czech Science Foundation

  • Programme

    Standard projects

  • Call for proposals

    Standardní projekty 6 (SGA02004GA-ST)

  • Main participants

  • Contest type

    VS - Public tender

  • Contract ID

Alternative language

  • Project name in Czech

    Insekticidní proteiny cílené proti trávicímu traktu hmyzu

  • Annotation in Czech

    Proteiny ovlivňující aktivitu a strukturu trávicího traktu hmyzu představují účinný prostředek k regulaci škůdců. Biologické, biochemické a molekulárně genetické metody budou použity při vyhledání nových molekul tohoto typu a charakterizaci jejich potenciálu při supresi škůdců řádu Coleoptera a Lepidoptera. Budou studovány insekticidní proteiny ovlivňující tři úrovně trávicího traktu: (i) hmyzí trávicí enzymy, proti kterým budou cíleny proteinové inhibitory s obrannou funkcí v rostlinách, (ii) střevní peritrofickou membránu, proti které budou cíleny rostlinné obranné chitinasy a proteiny vázající chitin, (iii) buňky střeva, proti kterým budou cíleny Bt-toxiny. Insekticidní potenciál studovaných proteinů bude na modelových hmyzích druzích určenpomocí (i) in vitro analysy distribuce trávicích enzymů a inhibice jejich aktivity, (ii) změny ve struktuře trávicího traktu určené mikroanatomií a imunodetekcí, (iii) in vivo testů s insekticidními proteiny podávanými v potravě s monitorováním

Scientific branches

  • R&D category

    ZV - Basic research

  • CEP classification - main branch

    GF - Diseases, pests, weeds and plant protection

  • CEP - secondary branch

    EI - Biotechnology and bionics

  • CEP - another secondary branch

    CE - Biochemistry

  • OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

    10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics

Completed project evaluation

  • Provider evaluation

    U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)

  • Project results evaluation

    The project was focused on proteins with insecticidal activity (bioinsecticides) directed against insect alimentary tract. First, we identified new bioinsecticides, determined their structure and inhibitory activity using biochemical methods (in vitro) a

Solution timeline

  • Realization period - beginning

    Jan 1, 2004

  • Realization period - end

    Jan 1, 2006

  • Project status

    U - Finished project

  • Latest support payment

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP07-GA0-GA-U/03:2

  • Data delivery date

    Oct 16, 2007

Finance

  • Total approved costs

    2,245 thou. CZK

  • Public financial support

    2,181 thou. CZK

  • Other public sources

    98 thou. CZK

  • Non public and foreign sources

    0 thou. CZK