Regulation of secondary metabolites biosynthesis in cell culture of Schisandra chinensis
Project goals
The intent of this project is the improvement of biotechnological methods designated for production of secondary metabolites with antioxidant activity, lignans and polyphenols, by plant cell cultures in vitro. The main objective of this study is the effect of precursors, elicitors and adsorbents on the production of antioxidant secondary metabolites by selected suspension cultures of Schisandra chinensis. It will be explored the impact of addition of different adsorbents, above all polymeric resins,on releasing of secondary metabolites from the cultured cells to media, on in situ adsorption of metabolites, on the overall increasing of its production and on possibility of continuous production of metabolites without harvesting the cells. The combined effects of precursors, elicitors and adsorbent will be also investigated as well as the biological activities of isolated secondary metabolites. Study of plant defense mechanisms connected with secondary metabolites production could help to
Keywords
cell cultureSchisandra chinensislignansprecursorselicitationadsorption
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
Standardní projekty 10 (SGA02007GA-ST)
Main participants
—
Contest type
VS - Public tender
Contract ID
522/07/0995
Alternative language
Project name in Czech
Regulace biosyntézy sekundarních metabolitů v buněčné kultuře Schisandra chinensis
Annotation in Czech
Záměrem tohoto projektu je zlepšení biotechnologických metod určených k produkci sekundárních metabolitů s antioxidační aktivitou, lignanů a polyfenolů, rostlinnými buněčnými liniemi in vitro. Hlavním cílem této studie je vliv prekurzorů, elicitorů a adsorbentů na produkci antioxidačních sekundárních metabolitů vybranými liniemi suzpenzních kultur Schisandra chinensis. Bude studován vliv přídavku různých adsorbentů, především polymerních pryskyřic, na uvolnění sekundárních metabolitů z kultivovaných buněk do media, na adsorpci metabolitů, na celkové zvýšení jejich produkce a na možnost kontinuální produkce metabolitů bez destrukce buněk. Společný vliv prekurzorů, elicitorů a adsorbentů bude rovněž studován stejně jako biologická aktivita izolovanýchsekundárních metabolitů. Studium obranných mechanismů rostlin, spojených s produkcí sekundárních metabolitů přispěje k optimalizaci kultivačních metod.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EI - Biotechnology and bionics
CEP - secondary branch
ED - Physiology
CEP - another secondary branch
CB - Analytical chemistry, separation
10406 - Analytical chemistry
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30105 - Physiology (including cytology)
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
.
Solution timeline
Realization period - beginning
Jan 1, 2007
Realization period - end
Dec 31, 2009
Project status
U - Finished project
Latest support payment
Apr 22, 2009
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP10-GA0-GA-U/02:2
Data delivery date
Jan 22, 2015
Finance
Total approved costs
3,286 thou. CZK
Public financial support
3,286 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Recognised costs
3 286 CZK thou.
Public support
3 286 CZK thou.
0%
Provider
Czech Science Foundation
CEP
EI - Biotechnology and bionics
Solution period
01. 01. 2007 - 31. 12. 2009