Sequencing and the genome analysis of the strawberry vein banding virus
Project goals
The project will bring original knowledge about the sequence and genome arrangement of the Strawberry vein banding virus (SVBV), which is a new virus recently found in the Europe. The virus will be isolated from infected strawberry plants, where the presence of SVBV will be confirmed by S-blotting. Viral nucleic acid will be isolated and fragmented by restriction endonucleases, cloned into plasmid following standard producers and sequenced. Sequence and the genome arrangement of the SVBV will be analysed and compared with other caulimoviruses. Its sequence will be screened and specific primers for routine diagnosis of the virus by PCR will be prepared. Promoter directed the synthesis of the whole virus genome transcript will be selected from the bank of clones and used in artificial construction to control the expression of ß-glucuronidase (GUS) marker gene.
Keywords
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
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Main participants
Ústav molekulární biologie rostlin AV ČR
Contest type
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Contract ID
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Alternative language
Project name in Czech
Sekvenování a analýza genomu viru lemování žilek jahodníku
Annotation in Czech
Projekt má přinést nové poznatky o sekvenci, uspořádání genomu a jeho regulačních elementů v Evropě nově popsaného viru lemování žilek jahodníku (Strawberry vein banding virus). Virus bude izolován z infikovaných rostlin jahodníku, ve kterých bude přítomnost SVBV potvrzena S-blottingem. Virová DNA bude standardními metodami fragmentována, klonována do pBS plasmidu a sekvenována. Získaná sekvence bude analyzována, porovnána se sekvencemi jiných caulimovirů a budou podle ní připraveny specifické púrimérypro rutinní diagnostiku viru metodou PCR. Z vytvořené banky klonů bude vybrán klon obsahující virový promotor řídící syntézu genomové mRNA. Tento promotor bude upraven restrikčním štěpením a začleněn do plasmidu, kde bude řídit expresi signálního genu beta-glucuronidázy (GUS).
Scientific branches
R&D category
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CEP classification - main branch
GE - Plant cultivation
CEP - secondary branch
EI - Biotechnology and bionics
CEP - another secondary branch
EB - Genetics and molecular biology
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30101 - Human genetics
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40105 - Horticulture, viticulture
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
Byla získána kompletní sekvence viru lemování žilek jahodníku. Nalezeny byly primery vhodné pro rutinní detekci viru pomocí PCR. Promotor viru byl začleněn do plazmidu, v němž kontroluje transkripci signálního genu beta-glukuronidázy. Charakteristika výs
Solution timeline
Realization period - beginning
Jan 1, 1996
Realization period - end
Jan 1, 1998
Project status
U - Finished project
Latest support payment
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Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP/1999/GA0/GA09GA/V/6:6
Data delivery date
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Finance
Total approved costs
1,796 thou. CZK
Public financial support
1,147 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
1 796 CZK thou.
Public support
1 147 CZK thou.
63%
Provider
Czech Science Foundation
CEP
GE - Plant cultivation
Solution period
01. 01. 1996 - 01. 01. 1998