Decoding Extracellular Protein–Protein Interactions with Synthetic Peptides

Project name in Czech
Decoding Extracellular Protein–Protein Interactions with Synthetic Peptides
Annotation in Czech
An average human body contains 1.5 kg of type I collagen—15% of all protein content. Collagen I is a structural scaffold and signaling hub, and plays a major role in fibrosis, with fibrotic diseases accounting for over 45% of deaths in the developed world. Understanding the interactions of collagen I with other biomolecules is thus of utmost importance for biomedical research. Despite its abundance and importance, the interactome of collagen I remains poorly understood. As a major obstacle, collagen I cannot be isolated as a pure and homogenous protein, impeding systematic studies. In this project, we will recreate the sequence and triple-helical structure of collagen I via a library of self-assembled heterotrimeric peptide fragments. Towards this goal, we will develop short domains that will trimerize the collagen fragments in a defined spatial arrangement. Screening of the library against extracellular and cell-surface proteins will allow us to map the collagen I interactome, providing unprecedented insight into the biology of one of the most abundant proteins in the human body.

R&D category
ZV - Basic research
OECD FORD - main branch
10401 - Organic chemistry
OECD FORD - secondary branch
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OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
CC - Organic chemistry

Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GM-R
Data delivery date
May 4, 2026

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