Plasmonic Nanoparticles Anchored Electrospun Fiber based SERS Nanotag for Multiplex Melanoma Biomarkers Detection via Antibody Targeted Immunoassay
Public support
Provider
Czech Science Foundation
Programme
—
Call for proposals
SGA0202600004
Main participants
Univerzita Palackého v Olomouci / Přírodovědecká fakulta
Contest type
VS - Public tender
Contract ID
26-23361I
Alternative language
Project name in Czech
Plasmonic Nanoparticles Anchored Electrospun Fiber based SERS Nanotag for Multiplex Melanoma Biomarkers Detection via Antibody Targeted Immunoassay
Annotation in Czech
The development of a novel electrospun fiber-based SERS nanotag platform designed for the multiplex detection of melanoma biomarkers is proposed. PLA electrospun fibers serve as solid supports for plasmonic nanoparticles, maximizing hotspot generation. The nanosystem is functionalized with 4-MBA and encapsulated in PEG/silica shells, enhancing stability against biochemical degradation and enabling bio-conjugation. Antibodies targeting three important melanoma biomarkers- CSPG4, Melan-A, and S100A8 are conjugated to the encapsulated NPs, enabling specific recognition and multiplex detection. The platform is validated through ultrasensitive detection of biomarkers at clinically relevant concentrations using SERS and in vitro studies with A375 melanoma cell lines. Bioimaging via confocal laser scanning microscopy will further assess biomarker interactions in normal skin cell lines, validating the platform’s applicability in complex biological matrices. This study holds promise as a novel, ultrasensitive, and cost-effective solution for early cancer diagnosis and monitoring.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)
OECD FORD - secondary branch
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OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
JJ - Other materials
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2028
Project status
Z - Beginning multi-year project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GN-R
Data delivery date
May 4, 2026
Finance
Total approved costs
4,760 thou. CZK
Public financial support
4,760 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK