Quantitative analysis of p16 gene as a potential prognostic marker of bladder transitional cell carcinomas
Project goals
Bladder cancer is the second most frequent malignancy among the tumours of the urinary tract in men - prostate carcinoma being the first. Extensive heterogeneity in tumour behaviour and the absence of accurate method for the early detection of tumour progression or relapse lead to the intensive search for new prognostic markers. The aim of the proposed project is to verify the possible use of p16 gene as a prognostic marker for the follow-up of patients with bladder transitional cell carcinomas. We willestablish very sensitive and exact approach: real-time quantitative polymerase chain reaction (RQ-PCR). We will compare p16 status (normal, hemizygous deletion, homozygous deletion) in a normal patient's tissue, tumour and urine specimen. We will trto define the relationship between this potential marker and histopathological and clinical features of the tumour as well as continuous tumour progression and/or relapse in the cohort of patients. Moreover, we will test DNA from the urine sediment as a
Keywords
Public support
Provider
Czech Science Foundation
Programme
Post-graduate (doctorate) grants
Call for proposals
Postdoktorandské granty 2 (SGA02002GA-PD)
Main participants
Univerzita Karlova / 2. lékařská fakulta
Contest type
VS - Public tender
Contract ID
—
Alternative language
Project name in Czech
Kvantitativnˇ stanovenˇ genu p16 jako mo§n?ho prognostick?ho markeru u uroteli lnˇch n dor? moźov?ho mŘchěýe
Annotation in Czech
N dory moźov?ho mŘchěýe jsou po karcinomu prostaty druhěm nejźastŘjçˇm urologickěm novotvarem u mu§?. Znaźn heterogenita v chov nˇ n dor? a absence spolehliv? metody pro vźasnou detekci n dorov? progrese źi recidivy vedou k intensivnˇmu hled nˇ nověch prognostickěch znak?. Cˇlem navrhovan?ho projektu je ovŘýit mo§nost vyu§itˇ n dorov?ho supresorov?ho genu p16 jako prognostick?ho markeru pýi sledov nˇ pacient? s uroteli lnˇmi n dory moźov?ho mŘchěýe. Bude zavedena kvantitativnˇ polymerasov ýetŘzov reakce v re ln?m źase (RQ-PCR), velice citliv a pýesn metoda pro kvantifikaci gen?. Status genu p16 (norm lnˇ stav, hemizygotnˇ nebo homozygotnˇ delece) bude porovn v n mezi norm lnˇ tk nˇ, n dorovou tk nˇ a vzorkem moźi konkr?tnˇho pacienta. D?le§itousouź stˇ projektu bude definov nˇ vztah? tohoto mo§n?ho markeru jak k histopatologickěm a klinickěm parametr?m tumoru, tak i k dalçˇ progresi źi recidivŘ n doru sledovaněch pacient?. D le budeme testovat DNA moźov?ho sedimentu jako mo§ně zdroj informacˇ
Scientific branches
R&D category
—
CEP classification - main branch
FD - Oncology and haematology
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
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10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
30101 - Human genetics
30204 - Oncology
30205 - Hematology
Completed project evaluation
Provider evaluation
N - Nesplněno zadání
Project results evaluation
Projekt byl pýedźasnŘ ukonźen na § dost ýeçitelky, vzhledem k n stupu na mateýskou dovolenou. Cˇlem projektu bylo vyu§ˇt alelickěch forem genu p 16 pro diagnostiku n dor? moźov?ho mŘchěýe. Jediněm pýˇnosem projektu bylo shrom §dŘnˇ klinick?ho materi lu p
Solution timeline
Realization period - beginning
Jan 1, 2001
Realization period - end
Jan 1, 2004
Project status
S - Stopped (prematurely terminated) multi-year project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP/2003/GA0/GA03GP/U/N/7:4
Data delivery date
Jun 15, 2009
Finance
Total approved costs
333 thou. CZK
Public financial support
333 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Recognised costs
333 CZK thou.
Public support
333 CZK thou.
0%
Provider
Czech Science Foundation
CEP
FD - Oncology and haematology
Solution period
01. 01. 2001 - 01. 01. 2004