Strategies for the improvement of secondary metabolite production in plant cell culture of Panax species
Project goals
Panax ginseng C. A. Meyer (Araliaceae) is a herbal plant which is widely known as a tonicum, adaptogen and geriatricum. Saponins (called ginsenosides) are major pharmacological compounds however later studies showed an important biologic activity of somenon-saponin compounds. Cytotoxic activity in vitro has been documented for polyacetylenes isolated from ginseng roots. Ginseng polysaccharides have been reported to inhibit growth of tumour cells in vivo. The aim of the project is a study of ginsenosideproduction in in vivo cultures of Panax ginseng through testing the effect of different stress factors, elicitors or precursors. The accumulation of ginsenosides and polyacetylenes will be compared in different cell (callus and suspension cultures) andorgan cultures (adventitious roots, somatic embryos, in vitro regenerated plantlets). On the basis of acquired results we would optimize the process of ginsenoside production by selection of the most suitable growth and production cultivation medium and
Keywords
Public support
Provider
Czech Science Foundation
Programme
Post-graduate (doctorate) grants
Call for proposals
Postdoktorandské granty 2 (SGA02002GA-PD)
Main participants
Ústav organické chemie a biochemie AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
—
Alternative language
Project name in Czech
Cesty ke zlepšení využitelnosti sekundárního metabolismu rostlin rodu Panax v in vitro kulturách
Annotation in Czech
Panax ginseng C. A. Meyer (Araliaceae) je bylina, která je široce známá jako tonizující rostlina a jako adaptogen a geriatrikum. Hlavními farmakologickými účinnými látkami jsou saponiny (ginsenosidy), ale novější studie prokázaly významnou biologickouaktivitu i u některých nesaponinových sloučenin. U polyacetylenů izolovaných z ženšenu byla zjištěna cytotoxická aktivita in vitro a některé polysacharidy izolované z kořene ženšenu inhibují růst rakovinových buněk in vivo. V tomto projektu se budemezabývatprodukcí ginsenosidů a polyacetylenů v in vitro kulturách Panax ginseng. Chtěli bychom studovat vliv různých stresových faktorů, elicitorů či prekursorů a porovnat akumulaci ginsenosidů a polyacetylenů v různých buněčných (kalusové a suspenzní) aorgánových kulturách (např.: adventivní kořínky, somatická embrya, regenerované rostlinky). Na základě těchto výsledků bychom chtěli optimalizovat proces produkce sekundárních metabolitů ženšenu výběrem vhodného růstového a produkčního kultivačního média
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EI - Biotechnology and bionics
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
CB - Analytical chemistry, separation
10406 - Analytical chemistry
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30101 - Human genetics
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
Our results showed that differentiated tissue as adventitious roots of Panax ginseng C.A. Meyer is optimal for production of ginseng pharmacologically important compounds. Adventitious root cultures, contrary to callus cultures (undifferentiated tissue),
Solution timeline
Realization period - beginning
Jan 1, 2002
Realization period - end
Jan 1, 2004
Project status
U - Finished project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP/2005/GA0/GA05GP/U/N/A:8
Data delivery date
Jul 23, 2008
Finance
Total approved costs
759 thou. CZK
Public financial support
663 thou. CZK
Other public sources
96 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
759 CZK thou.
Public support
663 CZK thou.
87%
Provider
Czech Science Foundation
CEP
EI - Biotechnology and bionics
Solution period
01. 01. 2002 - 01. 01. 2004