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Anti-inflammatory activity of plant quinones

Project goals

Scope of this project is the study inhibition of cyclooxygenases (COXs) and lipoxygenases (LOXs) enzymes by plant quinones. COXs and LOXs play key role in regulation of inflammatory diseases, in growth of cancer cells and in other pathological processesin mammalian tissues. Therefore currant research is aimed on searching for dual inhibitors of COX-2 and 5-LOX enzymes. Plant compounds are served as prospective source of these compounds. In vitro enzyme assay monitoring production of prostaglandins andleukotrienes which are products of COX and LOX pathway respectively will be used for tests. Screening of selected plant quinones for COX-1, COX-2 and 5-LOX inhibition and impact of simple derivatization steps on its activity will be carried out during the project solving. For perspective compounds inhibitory concentrations (IC50) will established and selectivity for enzymes will be calculated. Most active compounds will be tested for its toxicity. Moreover, study of mechanism of inhibition will be performed for selected constituents.

Keywords

rostlinné chinonycyklooxygenázalipoxygenázaléčivé rostliny

Public support

  • Provider

    Czech Science Foundation

  • Programme

    Post-graduate (doctorate) grants

  • Call for proposals

    Postdoktorandské granty 9 (SGA02009GA1PD)

  • Main participants

  • Contest type

    VS - Public tender

  • Contract ID

    525/09/P528

Alternative language

  • Project name in Czech

    Protizánětlivá aktivita rostlinných chinonů

  • Annotation in Czech

    Cílem projektu je nalezení látek efektivně inhibujících současně COX-2 a 5-LOX, které by mohly sloužit pro vývoj nových bezpečných protizánětlivých léků. Dalším cílem je ověřit vliv jednoduchých derivatizací chinonů na jejich inhibiční aktivitu. U aktivních látek zjištění způsobu inhibice enzymů.

Scientific branches

  • R&D category

    ZV - Basic research

  • CEP classification - main branch

    GM - Food industry

  • CEP - secondary branch

  • CEP - another secondary branch

  • 21101 - Food and beverages

Completed project evaluation

  • Provider evaluation

    U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)

  • Project results evaluation

    The project gone according to plan both in terms of expertise and in terms of disbursement of funds.

Solution timeline

  • Realization period - beginning

    Jan 1, 2009

  • Realization period - end

    Dec 31, 2011

  • Project status

    U - Finished project

  • Latest support payment

    Apr 16, 2011

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP12-GA0-GP-U/03:3

  • Data delivery date

    May 2, 2016

Finance

  • Total approved costs

    1,219 thou. CZK

  • Public financial support

    1,219 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    0 thou. CZK

Basic information

Recognised costs

1 219 CZK thou.

Public support

1 219 CZK thou.

100%


Provider

Czech Science Foundation

CEP

GM - Food industry

Solution period

01. 01. 2009 - 31. 12. 2011