Mechanisms of MPMV Nuclear Entry and Dynamics: Investigating Capsid Interactions, Host Pathways, and Core Mobility
Public support
Provider
Czech Science Foundation
Programme
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Call for proposals
SGA0202600006
Main participants
Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
Contest type
VS - Public tender
Contract ID
26-23154N
Alternative language
Project name in Czech
Mechanisms of MPMV Nuclear Entry and Dynamics: Investigating Capsid Interactions, Host Pathways, and Core Mobility
Annotation in Czech
Retroviruses such as HIV-1 replicate in the nucleus, but the nuclear entry mechanisms of other retroviruses remain poorly understood. This project focuses on Mason-Pfizer monkey virus (MPMV), which represents a promising and safer model for gene therapy, to investigate whether intact capsids transit through the nuclear pore and if subsequent nuclear actin-myosin transport is required for integration. Using super-resolution fluorescence and electron microscopy we will visualise GFP-tagged MPMV capsids in infected cells. We will use targeted mutagenesis of the capsid gene and known HIV-1 capsid inhibitors to determine whether MPMV shares pathways with HIV-1. Reverse transcriptase-deficient mutants will determine whether integration depends on reverse transcription before nuclear entry. By combining co-immunoprecipitation, mass spectrometry and cytoskeletal inhibition, we will map key virus-host nuclear interactions. These findings could reveal common retroviral import strategies, and provide insight into the design and development of more efficient retroviral vectors for gene therapy.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10607 - Virology
OECD FORD - secondary branch
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OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
EE - Microbiology, virology
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2029
Project status
Z - Beginning multi-year project
Latest support payment
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Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GY-R
Data delivery date
Apr 27, 2026
Finance
Total approved costs
4,645 thou. CZK
Public financial support
4,231 thou. CZK
Other public sources
345 thou. CZK
Non public and foreign sources
69 thou. CZK