Expression of heterologous membrane carriers in yeast

Provider

Academy of Sciences of the Czech Republic

Programme

Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Republic

Call for proposals

—

Main participants

Fyziologický ústav AV ČR, v. v. i.

Contest type

—

Contract ID

—

Project name in Czech

Exprese heterologních membránových přenášečů v kvasinkách

Annotation in Czech

Bude sledována exprese, aktivita a degradace heterologních přenášečů bazických aminokyselin v kvasince Saccharomyces cerevisiae. Geny příslušných přenášečů (permeáz) (z Candida albicans a Schirosaccharomyces pombe) budou do S. cerevisiae klonovány funkční komplementací a pak bude sledována posttranslační cesta jejich produktů do membrány (využití sec-mutantů), transportní aktivita těchto heterologních systémů, jakož i jejich regulace a degradace. Vše se bude provádět paralelně ve srovnání s vlastními, rovněž však klonovanými transportními systémy kvasinky S. cerevisiae, a to jak konstitutivními (přenášeč pro lysin) tak regulovatelnými (přenášeč pro galaktosu).

R&D category

—

CEP classification - main branch

EE - Microbiology, virology

CEP - secondary branch

EI - Biotechnology and bionics

CEP - another secondary branch

—

OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

10606 - Microbiology<br>10607 - Virology<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics

Realization period - beginning

Jan 1, 1994

Realization period - end

Jan 1, 1996

Project status

U - Finished project

Latest support payment

—

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data delivery code

CEP/1997/AV0/AV07IA/V/9:4

Data delivery date

—

Total approved costs

329 thou. CZK

Public financial support

503 thou. CZK

Other public sources

0 thou. CZK

Non public and foreign sources

0 thou. CZK