Characterization of the Fur phenotype of mutants of Pseudomonas aeruginosa: over-production of components of iron transport system.
Public support
Provider
Academy of Sciences of the Czech Republic
Programme
The research grant projects for juniors
Call for proposals
Juniorské badatelské grantové projekty 5 (SAV02007-B)
Main participants
—
Contest type
VS - Public tender
Contract ID
KJB500200703
Alternative language
Project name in Czech
Charakterizace Fur fenotypu mutantů Pseudomonas aeruginosa: nadprodukce komponent transportního systému pro FeIII.
Annotation in Czech
Projekt se zabývá charakterizací transportního mechanismu pro FeIII, jednoho z faktorů virulence, u Fur mutantů P. aeruginosa a možným využitím těchto poznatků v biotechnologiích. Projekt řeší následující cíle: 1. vztah mezi nadprodukcí pyoverdinu a jehospecifického receptoru u Fur mutantů, 2. maximální biosyntetickou kapacitu pro komponenty FeIII-transportního systému v rekombinantních kmenech a Fur mutantech a 3. charakterizaci změn ve Fur regulonu u rodičovského a Fur fenotypu. Metodami rekombinantní DNK budou připraveny rekombinantní kmeny obou fenotypů, u nichž bude sledována nadprodukce komponent FeIII-transportního systému za definovaných kultivačních podmínek v míchaném bioreaktoru. Pomocí metody DNA microarray budou studovány změny ve Fur regulonu u rodičovského a Fur fenotypu jako odpověď buněk na přítomnost vysoké koncentrace FeIII v mediu. Nadprodukované komponenty transportního systému lze využít v medicíně, farmacii a dalších oblastech vědy a výzkumu.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EE - Microbiology, virology
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
EI - Biotechnology and bionics
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10606 - Microbiology<br>10607 - Virology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30101 - Human genetics<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
This project deals with characterization of fur mutants FF13 and FPA12 derived from strain Pseudomonas aeruginosa in terms of global regulation of Fur regulon and maximum biosynthetic capacity for siderophores and their specific receptors FpvA and FptA.
Solution timeline
Realization period - beginning
Jan 1, 2007
Realization period - end
Dec 31, 2009
Project status
U - Finished project
Latest support payment
Apr 28, 2009
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP10-AV0-KJ-U/03:3
Data delivery date
Jun 14, 2011
Finance
Total approved costs
1,258 thou. CZK
Public financial support
1,258 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK