Regulation of Transcription of Genes Colding for Light-harvesting Proteins in Phytoplankton under Fluctuating Light Conditions
Project goals
Photosynthesis is regulated at different levels under influence of fluctuating light intensity, temperature and nutrient availability. One of the regulation level are changes in cellular pigment content, associated with the synthesis and degradation of proteins of light-harvesting antennae. These can be divided accordint to the associated secondary pigments (LHC for chla/b,FCP for chla/c and fucoxanthin, PCP for chl a and peridinine). Light-harvesting antennae are composed from several homologous proteins encoded in nucleus. Although the exact reasons for multiple LHC proteins are not known, it is assumed thet each of those proteins has distinct role. Only little is known about a) light-harvesting regulation mechanisms in fluctuating light environments, b) regulation of LHC, FCP a PCP complexes, c) regulation of particular homologous proteins. The proposed project will focus on answerint these questions using modern methods of molecular biology.
Keywords
light-harvesting complexesfluctuating lightgene experssionpigmentsLHCFCPPCPhomologous light-harvesting protein familyredox sensorsplastoquinon poolthioredoxin/ferrodoxin cascadevariable fluorescence
Public support
Provider
Academy of Sciences of the Czech Republic
Programme
The research grant projects for juniors
Call for proposals
Juniorské badatelské grantové projekty 3 (SAV02005-J)
Main participants
—
Contest type
VS - Public tender
Contract ID
KJB600200515
Alternative language
Project name in Czech
Regulace genů kódujících proteiny fotosyntetických antén u řas v proměnném světelném prostředí
Annotation in Czech
Fotosyntéza je regulována na různých úrovních v závislosti na intenzitě fluktuujících světla, teploty a dostupnosti živin. Jednou z úrovní regulace je změna buněčné pigmentace, spojená se syntézou a degradací proteinů světlosběrných antén. Světlosběrné anténní komplexy eukaryotních řas se rozdělují podle toho, jaké sekundární pigmenty jsou v anténách navázány (LHC pro chla/b, FCP pro chla/c a fucoxanthin, PCP u chla a peridininu). Světlosběrné antény obsahují několik homologních proteinů kódovaných v jádře. Ačkoliv přesné důvody pro multiplicitu LHC proteinů nejsou dosud jasné, předpokládá se, že každý z těchto proteinů má jednoznačnou funkci. Téměř nic není známo a) o mechanismech regulace světlosběrných antén v přirozených podmínkách proměnné intenzity ozářenosti, b) o rozdílech v regulaci LHC, FCP a PCP komplexů, c) o regulaci jednotlivých homologních proteinů. Tyto problémy budeme v navrhovaném projektu studovat pomocí moderních metod molekulární biologie.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
EE - Microbiology, virology
CEP - another secondary branch
EI - Biotechnology and bionics
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10606 - Microbiology
10607 - Virology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30101 - Human genetics
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
O - Nesplněno zadání, smlouva však byla dodržena
Project results evaluation
While some partial succes in the experimental part of the project has been reached, the results of the project have not been published in any scientific periodical as yet.
Solution timeline
Realization period - beginning
Jan 1, 2005
Realization period - end
Dec 1, 2007
Project status
U - Finished project
Latest support payment
Feb 27, 2007
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP08-AV0-KJ-U/02:2
Data delivery date
Apr 3, 2009
Finance
Total approved costs
1,790 thou. CZK
Public financial support
1,790 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Recognised costs
1 790 CZK thou.
Public support
1 790 CZK thou.
0%
Provider
Academy of Sciences of the Czech Republic
CEP
EB - Genetics and molecular biology
Solution period
01. 01. 2005 - 01. 12. 2007