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LD12073

Membrane signalosomes of mast cells and basophils - targets for innovative therapies

Project goals

The aim of this project is: 1. To contribute to determining the composition of the FceRI membrane signalosomes important for antigen-induced degranulation in mast cells. 2. To contribute to determining the composition of membrane signalosomes important for antigen-induced adhesion and chemotaxis in mast cells. 3. To determine new modulators (inhibitors/activators) of antigen-induced degranulation, adhesion and chemotaxis in mouse and human mast cells.

Keywords

mast cellIgE receptorimmunoreceptor-induced activationshRNAlentiviral transduction

Public support

  • Provider

    Ministry of Education, Youth and Sports

  • Programme

    COST CZ

  • Call for proposals

    COST CZ 2 (SMSM2012LD2)

  • Main participants

  • Contest type

    VS - Public tender

  • Contract ID

    MSMT-2/2013-311

Alternative language

  • Project name in Czech

    Membránové signalosomy žírných buněk a bazofilů - cíle pro inovativní terapie

  • Annotation in Czech

    Cílem projektu je 1. Přispět k určení složení FceRI membránových signalosomů důležitých pro degranulaci žírných buněk. 2. Přispět k určení složení membránových signalosomů důležitých pro antigenem indukovanou adhezi a chemotaxi u žírných buněk 3. Určit nové modulátory (inhibitory/aktivátory) antigenem indukované adheze, chemotaxe a degranulace u myších a lidských žírných buněk.

Scientific branches

  • R&D category

    ZV - Basic research

  • CEP classification - main branch

    EC - Immunology

  • CEP - secondary branch

    EB - Genetics and molecular biology

  • CEP - another secondary branch

    ED - Physiology

  • 10603 - Genetics and heredity (medical genetics to be 3)
    10604 - Reproductive biology (medical aspects to be 3)
    10605 - Developmental biology
    10608 - Biochemistry and molecular biology
    10609 - Biochemical research methods
    30101 - Human genetics
    30102 - Immunology
    30105 - Physiology (including cytology)

Completed project evaluation

  • Provider evaluation

    V - Vynikající výsledky projektu (s mezinárodním významem atd.)

  • Project results evaluation

    Results of this project contributed to the discovery of new biological functions of three plasma membrane proteins (tetraspanin CD9 and transmembrane adaptor proteins PAG and NTAL) in mast cell degranulation, adhesion and chemotaxis. Furthermore, the results showed that plasma membrane cholesterol in activated mast cells is involved in regulating sensitivity towards ethanol..

Solution timeline

  • Realization period - beginning

    Mar 1, 2012

  • Realization period - end

    Dec 31, 2014

  • Project status

    U - Finished project

  • Latest support payment

    Mar 3, 2014

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP15-MSM-LD-U/01:1

  • Data delivery date

    Jul 2, 2015

Finance

  • Total approved costs

    1,500 thou. CZK

  • Public financial support

    1,500 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    0 thou. CZK

Recognised costs

1 500 CZK thou.

Public support

1 500 CZK thou.

0%


Provider

Ministry of Education, Youth and Sports

CEP

EC - Immunology

Solution period

01. 03. 2012 - 31. 12. 2014