Analysis of DNA and Protein Damage Involved by ROS
Public support
Provider
Ministry of Education, Youth and Sports
Programme
COST CZ
Call for proposals
COST CZ 4 (SMSM2014LD4)
Main participants
Univerzita Palackého v Olomouci / Lékařská fakulta
Contest type
VS - Public tender
Contract ID
MSMT-8634/2014-1
Alternative language
Project name in Czech
Analýza poškození DNA a proteinů navozeného ROS
Annotation in Czech
Hlavním cílem projektu je tedy přinést nové metodické postupy pro výzkum interakce ROS a využít tyto postupy k výzkumu interakce membránových proteinů, které sehrávají významnou úlohu v procesu regulace energetického metabolismu (UCP1), transportu iontů(Na/K ATPasa a KtrAB) a buněčném dělení a virulenci bakterií (FTT 1103).
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
CE - Biochemistry
CEP - secondary branch
BO - Biophysics
CEP - another secondary branch
CG - Electrochemistry
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>10610 - Biophysics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
During time period 2014-2016, isolation and purification protocols for cytochrome c and its apoform/porphyrine-form, protein FTT1103, Na/K ATPase, UCP1 protein and SERCA were developed and optimized. The proteins were studied using electrochemical methods and, protocol for measurement of protein glycation was published. EPR analyses of protein redox status were realized via COST EU-ROS (BMBS - Biomedicine and Molecular Biosciences - BM1203) action. Finally, we prepared proteoliposomes using Na/K ATPase for consecutive redox properties evaluation.
Solution timeline
Realization period - beginning
Apr 1, 2014
Realization period - end
Nov 30, 2016
Project status
U - Finished project
Latest support payment
Feb 12, 2016
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP17-MSM-LD-U/01:1
Data delivery date
Jun 23, 2017
Finance
Total approved costs
1,930 thou. CZK
Public financial support
1,930 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK