Chemoprotective and biological activities of the new dehydro-derivatives of flavonolignans of silymarine.
Public support
Provider
Ministry of Education, Youth and Sports
Programme
COST CZ
Call for proposals
COST CZ 4 (SMSM2014LD4)
Main participants
Mikrobiologický ústav AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-8634/2014-1
Alternative language
Project name in Czech
Chemoprotektivní a biologické aktivity nových dehydro-derivátů flavonolignanů silymarinu.
Annotation in Czech
Bude vypracována preparativní metoda k získání multigramových množství opticky čistých výchozích látek silychristinu a silydianinu. Budou vypracovány metody šetrné oxidace silychristinu a silydianinu s využitím chemických a chemoenzymatických metod (např. oxidasy a laccasy) vedoucí k dosud nepopsaným látkám 2,3-dehydrosilychristinu a 2,3-dehydrosilydianinu v opticky čistém stavu. Budou též připraveny nové deriváty těchto sloučenin. U těchto nových látek budou především testovány antioxidační, protiradikálové a redoxní účinky a dále schopnosti komplexace přechodných kovů účastnících se Fentonovy reakce, která vede k oxidačnímu stresu. Ve spolupráci s členy projektu COST budou vybrané nové látky testovány v biologických systémech (in vitro a ex vivo) promožnosti antioxidační ochrany proteinů, případně dalších biomakromolekul.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
CE - Biochemistry
CEP - secondary branch
EI - Biotechnology and bionics
CEP - another secondary branch
CC - Organic chemistry
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10401 - Organic chemistry<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
"We have developed a method for the preparation of dehydroderivatives of silychristin and silydianin based on the oxidation in pyridine. New compounds were characterized from the point of antioxidant, antiradical and electrochemical activities. We developed an enzymatic method for sulfated metabolites synthesis, which will be used for silychristin metabolite preparation. "
Solution timeline
Realization period - beginning
Apr 1, 2014
Realization period - end
Dec 31, 2016
Project status
U - Finished project
Latest support payment
Feb 12, 2016
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP17-MSM-LD-U/01:1
Data delivery date
Jun 23, 2017
Finance
Total approved costs
2,099 thou. CZK
Public financial support
2,015 thou. CZK
Other public sources
84 thou. CZK
Non public and foreign sources
0 thou. CZK