Fluorinated carbohydrates: A valuable tool to study glycan-protein interactions and remodel cell-surface glycome
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
SMSM2020LTC01
Main participants
Ústav chemických procesů AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-21080/2020-26
Alternative language
Project name in Czech
Fluorinated carbohydrates: A valuable tool to study glycan-protein interactions and remodel cell-surface glycome
Annotation in Czech
The objectives of the project are divided into three areas according to their scientific nature. 1) Synthesis. The objectives in this area are 1a) The synthesis of a complete library of systematically deoxofluorinated deprotected analogs of LacNAc, LacdiNAc and diacetylchitobiose ready for determination of binding affinity, and for NMR epitope mapping; 1b) The synthesis of systematically deoxofluorinated GlcNAc, GalNAc, and ManNAc protected at hydroxyl groups as acetyl, propionyl and butyryl esters; 1c) Preparation of a limited number of protected disaccharide units of GAGs intended as a demonstration of the feasibility of this first step towards assembly of functional segments of fluorinated GAG. 2) Ligand recognition. The objectives in this area are 2a) Determination of the binding affinity of deoxofluorinated LacNAc, LacdiNAc and diacetylchitobiose analogs towards their cognate lectins by ELISA and ELLA assays; 2b) Epitope mapping of LacNAc, LacdiNAc and diacetylchitobiose using advanced NMR techniques. 3) Metabolic inhibition. The objectives in this area are 3a) Identification of deoxofluorinated acylated hexosamines usable for manipulation of cell-surface glycome by metabolic inhibition; 3b) Determination of how the cell-surface glycome has been altered after treatment with compounds identified under 3a.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10401 - Organic chemistry
OECD FORD - secondary branch
10608 - Biochemistry and molecular biology
OECD FORD - another secondary branch
—
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
CC - Organic chemistry<br>CE - Biochemistry<br>EB - Genetics and molecular biology
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
The project included synthesis and lectin affinities of three complete series of fluorinated disaccharides, synthesis and cytotoxicity of fluorinated amino saccharides, their organometallic conjugates, NMR analysis of interactions with galectin-1 and -3, and conformational analysis. Three publications in WoS-indexed journals were published and two more are being prepared for submission.
Solution timeline
Realization period - beginning
May 15, 2020
Realization period - end
Apr 7, 2023
Project status
U - Finished project
Latest support payment
Feb 17, 2023
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP24-MSM-LT-U
Data delivery date
Jul 1, 2024
Finance
Total approved costs
4,119 thou. CZK
Public financial support
4,119 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK