Nuclear regulatory landscape of bZIP transcription factors in plant reproductive development (REPROZIP)
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
SMSM2020LTC01
Main participants
Ústav experimentální botaniky AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-21080/2020-16
Alternative language
Project name in Czech
Nuclear regulatory landscape of bZIP transcription factors in plant reproductive development (REPROZIP)
Annotation in Czech
The principal objective of this project proposal is to decipher and visualise the complexity of pollen Group E/I bZIP TF nuclear regulatory landscape in relation to chromatin dynamics during Arabidopsis pollen development and under the effect of environmental cues. For this, we will take the advantage of our model material being effectively single cell system – naturally synchronised population of developing spores and growing pollen tubes. To demonstrate the function of bZIP regulatory landscape in pollen it will be necessary to visualise bZIP proteins and their interactions and to identify their downstream pathways, target genes and binding sites. This will be achieved by the functional characterization of bzip double mutants and by the combination of transcriptomic (RNA-seq), proteomic (LC-MS/MS) and lipidomic (LC-MS) approaches that will be compared to the previously published bzip34 and bzip18 microarray data. As all bZIP genes of interest were demonstrated to interact in the Y2H assay, we aim to clarify whether they co-operate on the same regulatory pathway(s) and whether they form native functional and/or regulatory complexes.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10611 - Plant sciences, botany
OECD FORD - secondary branch
—
OECD FORD - another secondary branch
—
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
EF - Botany
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project has successfully terminated
Solution timeline
Realization period - beginning
Jun 1, 2020
Realization period - end
May 12, 2023
Project status
U - Finished project
Latest support payment
Feb 17, 2023
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP24-MSM-LT-U
Data delivery date
Jul 1, 2024
Finance
Total approved costs
5,272 thou. CZK
Public financial support
5,272 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK