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Role of adhesion-GPCRs during viral infection of mammalian cells

Public support

  • Provider

    Ministry of Education, Youth and Sports

  • Programme

    INTER-EXCELLENCE

  • Call for proposals

    SMSM2020LTC01

  • Main participants

    Ústav organické chemie a biochemie AV ČR, v. v. i.

  • Contest type

    VS - Public tender

  • Contract ID

    MSMT-21080/2020-32

Alternative language

  • Project name in Czech

    Role of adhesion-GPCRs during viral infection of mammalian cells

  • Annotation in Czech

    Cíle projektu zahrnují: - analyzovat změny v expresi aGPCR a / nebo proteinů zapojených do aGPCR drah mezi infikovanými a neinfikovanými buňkami pomocí hmotnostní spektrometrie -vyhodnotit účinek vybraných aGPCR a / nebo proteinů zapojených do aGPCR drah na replikaci viru - Identifikovat a charakterizovat virové proteiny interagující s aGPCR a / nebo proteiny zapojenými do aGPCR drah.

Scientific branches

  • R&D category

    ZV - Basic research

  • OECD FORD - main branch

    10607 - Virology

  • OECD FORD - secondary branch

    10608 - Biochemistry and molecular biology

  • OECD FORD - another secondary branch

  • CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

    CE - Biochemistry<br>EB - Genetics and molecular biology<br>EE - Microbiology, virology

Completed project evaluation

  • Provider evaluation

    V - Vynikající výsledky projektu (s mezinárodním významem atd.)

  • Project results evaluation

    We determined upregulated expression of two adhesion GPCRs in lung cells during SARS-CoV-2 infection. After downregulation of their endogenous levels in lung cells we observed reduction in SARS-CoV-2 replication and infectivity. Thereby we accomplished main goal of our project to show that aGPCRs may have a role during viral infections.

Solution timeline

  • Realization period - beginning

    Jun 1, 2020

  • Realization period - end

    Oct 31, 2023

  • Project status

    U - Finished project

  • Latest support payment

    Feb 17, 2023

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP24-MSM-LT-U

  • Data delivery date

    Jul 1, 2024

Finance

  • Total approved costs

    5,951 thou. CZK

  • Public financial support

    4,752 thou. CZK

  • Other public sources

    1,199 thou. CZK

  • Non public and foreign sources

    0 thou. CZK