Library of engineered glycosidases for the preparation of bioactive glycans
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
SMSM2020LTC01
Main participants
Mikrobiologický ústav AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-21080/2020-38
Alternative language
Project name in Czech
Library of engineered glycosidases for the preparation of bioactive glycans
Annotation in Czech
The aim of this project is to construct a toolbox of genetically modified glycosidases with desired catalytic properties, in particular enhanced synthetic capability, enhanced regioselectivity and/ or substrate specificity, and to use these enzymes in cascade transglycosylation reactions to afford a library of tailored oligosaccharide ligands of biomedically relevant galectins. Due to the broad substrate specificity of engineered glycosidases, the prepared tailored oligosaccharides will bear suitable linkers for conjugation to multivalent biocompatible water-soluble carriers that will afford multivalent glycoconjugates with high affinity (avidity) and possibly also high selectivity for particular galectins. Affinity of prepared glycostructures will be tested by ELISA-type assays and other methods. The resulting glycoconjugates will be prospective for application in the therapy or prevention of diseases linked to galectin overexpression, especially tumor progression
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10608 - Biochemistry and molecular biology
OECD FORD - secondary branch
10401 - Organic chemistry
OECD FORD - another secondary branch
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
CC - Organic chemistry<br>CE - Biochemistry<br>EB - Genetics and molecular biology<br>EI - Biotechnology and bionics
Solution timeline
Realization period - beginning
Jun 1, 2020
Realization period - end
Apr 6, 2023
Project status
K - Ending multi-year project
Latest support payment
Feb 25, 2022
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP23-MSM-LT-R
Data delivery date
Oct 17, 2023
Finance
Total approved costs
3,997 thou. CZK
Public financial support
3,997 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK