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ME 433

Genetic engineering and molecular biology in grain legumes

Public support

  • Provider

    Ministry of Education, Youth and Sports

  • Programme

    KONTAKT

  • Call for proposals

    KONTAKT 1 (SMSM200132001)

  • Main participants

    AGRITEC, výzkum, šlechtění a služby, s.r.o.

  • Contest type

    VS - Public tender

  • Contract ID

Alternative language

  • Project name in Czech

    Využití metod genového inženýrství a molekulární biologie u luskovin

  • Annotation in Czech

    Cílem projektu je vývoj účinných metod genové transformace hrachu (Pisum sativum L.) a sóje (Glycine max (L.) Merr.) pomocí Agrobacterium tumefaciens nebo metodou biolistické transformace. Budou použity modelové konstrukty obsahující selekční markery a reportérové geny. Dále budou využity konstrukty obsahující užitečné geny (na konci tohoto projektu nebo jako pokračování projektu). Bude prováděna optimalizace regeneračních protokolů použitých pro transformaci u obou plodin, dále pak důkaz transformace uprimárních transformantů a jejich semenných potomstev metodami molekulární biologie. Studována bude také variabilita způsobená manipulacemi in vitro (somaklonální variabilita).

Scientific branches

  • R&D category

  • CEP classification - main branch

    GE - Plant cultivation

  • CEP - secondary branch

    EI - Biotechnology and bionics

  • CEP - another secondary branch

    EB - Genetics and molecular biology

  • OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

    10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30101 - Human genetics<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40105 - Horticulture, viticulture<br>40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics

Completed project evaluation

  • Provider evaluation

    V - Vynikající výsledky projektu (s mezinárodním významem atd.)

  • Project results evaluation

    Vytvořena metoda agrobakteriální transformace hrachu, potvrzeno zabudování transgenů do genomu hrachu a jejich přenos na potomstvo.Zjištěny nepravidelnosti v přenosu transgenů napotomstvo transgenních rostlin.Zprac. metodické základy biolit.transf.hrachu

Solution timeline

  • Realization period - beginning

    Jan 1, 2001

  • Realization period - end

    Jan 1, 2003

  • Project status

    U - Finished project

  • Latest support payment

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP/2004/MSM/MSM4ME/U/N/4:2

  • Data delivery date

    Jun 3, 2008

Finance

  • Total approved costs

    2,120 thou. CZK

  • Public financial support

    755 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    1,365 thou. CZK